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Panel:   AR Bank Custom set (Custom)
AR Bank # 0215 Staphylococcus aureus
Study ID: VISA-01

Biosample Accession #: SAMN04901605

   
Source: Wound

Panel:  Vancomycin Intermediate Staphylococcus aureus (VISA)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Cefoxitin 2>16R
Ceftaroline 1S
Clindamycin >16R
Daptomycin 2NS
Doxycycline <=1S
Erythromycin >8R
Gentamicin <=2S
Levofloxacin >16R
Linezolid 4S
Mupirocin <=4---
Oxacillin >16R
Penicillin >2R
Rifampin <=0.5S
Tetracycline <=1S
Trimethoprim/sulfamethoxazole 1<=0.5S
Vancomycin 4I
S – I –R Interpretation (INT) derived from CLSI 2021 M100 S31
NS = Nonsusceptible
1 Reflects MIC of first component
2 Cefoxitin is tested as a surrogate for mecA-mediated oxacillin resistance
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aph-STPH, spc
Beta-lactam DHA-1, mecA
Macrolide-Lincosamide-Streptogramin erm(A)
Tetracyclines tet(38)
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
08/01/22Gene was added
08/01/22Category was added
08/01/22Gene was added
08/01/22Gene was removed
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