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Panel:   AR Bank Custom set (Custom)
AR Bank # 0412 Campylobacter jejuni
Study ID: GI-12

Biosample Accession #: SAMN12648962

   
Source:
stool

Panel:  Enteric Pathogen Diversity (GI)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Azithromycin 0.03WT
Ciprofloxacin 0.5S
Erythromycin 0.5S
Florfenicol 4WT
Gentamicin 0.5WT
Nalidixic acid 8WT
Tetracycline >64R
S – I –R Interpretation (INT) derived from M45 2016 S3
WT (wild type; =ECVs) – NWT (non-wild type; =ECVs) based on epidemiological cutoff values (ECVs) adopted from the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2021)
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Tetracyclines tet(O)
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP); Brain Heart Infusion Agar with 5% Rabbit Blood (BHIA)

GROWTH CONDITIONS
Temperature: 37°C
Atmosphere: Micro-aerobic

PROPAGATION PROCEDURE
Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
Open vial aseptically to avoid contamination
Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
Aseptically transfer the loop to BHIA
Use streak plate method to isolate single colonies
Incubate inverted plate at 37°C for 24-48 hrs., then subculture to BAP and allow 48 hours of incubation prior to performing Antimicrobial Susceptibility Testing (AST).
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
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