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Panel:   AR Bank Custom set (Custom)
AR Bank # 0508 Pseudomonas aeruginosa
Study ID: IMR-08

Biosample Accession #: SAMN11953951
MLST: 235(Pasteur)

Panel:  Imipenem/relebactam (IMR)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin 32I
Aztreonam >64R
Cefepime 32R
Ceftazidime >128R
Ceftazidime/avibactam 1>16R
Ceftolozane/tazobactam 1>16R
Ciprofloxacin 4R
Colistin 20.5I
Doripenem >8R
Imipenem 32R
Imipenem/relebactam 18R
Imipenem+chelators 316---
Levofloxacin 8R
Meropenem >8R
Piperacillin/tazobactam 132I
Tobramycin >16R
SDD (Susceptible Dose Dependent)
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
1 Reflects MIC of first component
2 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
3 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aadA2, ant(2")-la, aph(3')-VIb, aph(3')-XV, strA
Beta-lactam OXA-2, PER-1
Phenicols/Bicyclomycins cmxA
Sulfonamides sul1
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
06/14/23Drug was removed: 'Gentamicin'
02/13/23MLST was updated: from '---' to '235(Pasteur)'
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