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Panel:   ARBank custom (n=29) (Custom)
AR Bank # 0055 Escherichia coli
Study ID: CarbaNP-24

Biosample Accession #: SAMN04014896
MLST: ST43(Pasteur), ST131(Achtman)

Panel:  Gram Negative Carbapenemase Detection (CarbaNP)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin >64R
Ampicillin >32---
Ampicillin/sulbactam 1>32R
Aztreonam 32R
Cefazolin >8R
Cefepime >32R
Cefotaxime >128R
Cefotaxime/clavulanic acid 1>32---
Cefoxitin >16R
Ceftazidime >128R
Ceftazidime/avibactam 1>16R
Ceftazidime/clavulanic acid 1>64---
Ceftolozane/tazobactam 1>16R
Ceftriaxone >32R
Ciprofloxacin >8R
Colistin 41I
Doripenem >8R
Ertapenem >8R
Gentamicin >16R
Imipenem 8R
Imipenem/relebactam 18R
Imipenem+chelators 3<=0.25---
Levofloxacin >8R
Meropenem >8R
Minocycline <=4S
Piperacillin/tazobactam 1>128R
Tetracycline >32R
Tigecycline 21S
Tobramycin >16R
Trimethoprim/sulfamethoxazole 1>8R
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 Reflects MIC of first component
2 Based on FDA break points
3 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
4 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(3)-IIa, aac(6')-Ib-D181Y, aadA5, rmtC
Beta-lactam NDM-1, CMY-6, OXA-1
Bleomycin ble-MBL
Efflux pumps/Other ACRF, qacEdelta1
Macrolide-Lincosamide-Streptogramin mph(A)
Phenicols/Bicyclomycins catB4
Sulfonamides sul1
Tetracyclines tet(A), tet(R)
Trimethoprim dfrA17
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
03/13/23Gene was added to 'qacEdelta1'
03/13/23Gene was added to 'ACRF'
03/13/23Gene was added to 'ble-MBL'
03/13/23Gene was added to 'catB4'
03/13/23Gene was added to 'tet(R)'
03/13/23Gene was added to 'aac(6')-Ib-D181Y'
03/13/23Category was added: 'Efflux pumps/Other'
03/13/23Category was added: 'Bleomycin'
03/13/23Category was added: 'Phenicols/Bicyclomycins'
12/19/22MLST was updated: from '---' to 'ST131(Achtman)'
12/19/22MLST was updated: from '---' to 'ST43(Pasteur)'
10/25/22INT was updated with a new value for Minocycline: from '---' to 'S'
10/25/22INT was updated with a new value for Imipenem/relebactam: from '---' to 'R'
10/25/22INT was updated with a new value for Ceftolozane/tazobactam: from '---' to 'R'
10/25/22MIC was updated with a new value for Minocycline: from '---' to '4'
10/25/22MIC was updated with a new value for Imipenem/relebactam: from '---' to '8'
10/25/22MIC was updated with a new value for Ceftolozane/tazobactam: from '---' to '16'
10/25/22Drug was added: Minocycline
10/25/22Drug was added: Imipenem/relebactam
10/25/22Drug was added: Ceftolozane/tazobactam
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