Skip directly to site content
Are You Still There?
Due to inactivity, you will be signed out in two minutes unless you click 'Continue'.

Are you still there?

Due to inactivity, you will be signed out in two minutes unless
you click 'Continue'.

Panel:   AR Bank Custom set (Custom)
AR Bank # 0572 Enterococcus faecium
Study ID: ATR-32

Biosample Accession #: SAMN11954004
MLST: 412(Pasteur)

Panel:  Aminoglycoside/tetracycline Resistance (ATR)  |  Aminoglycoside/tetracycline Resistance - Gram positive set (ATR-GP) (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin >128R
Chloramphenicol 16I
Daptomycin 4SDD
Doxycycline 16R
Eravacycline 10.03S
High-Level Gentamicin <=500SYN-S
High-Level Streptomycin <=1000SYN-S
Levofloxacin >8R
Linezolid 4I
Penicillin >256R
Quinupristin/dalfopristin 20.5S
Rifampin >4R
Teicoplanin >128R
Vancomycin 512R
S – I –R Interpretation (INT) derived from CLSI 2020 M100 S30
SDD (Susceptible Dose Dependent)
1 Based on FDA break points
2 Reflects MIC of the combination
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aacA-ENT1, aph(3')-IIIa
Glycopeptides VanA
Macrolide-Lincosamide-Streptogramin erm(B)
Streptothricin sat4
Trimethoprim dfrG
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
08/22/23INT was updated with a new value for Quinupristin/dalfopristin: from 'R' to 'S'
03/09/23MIC was updated with a new value for Eravacycline: from '0.06' to '0.03'
11/07/22MLST was updated
09/19/22Gene was added
09/19/22Category was added
09/19/22Gene was removed
Top of Page
TOP