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Panel:   Tedizolid/Linezolid (AR-0781 - 0795) and VRE (AR-0474 - 0488) (Custom)
AR Bank # 0785 Enterococcus faecalis
Study ID: VRE-05

Biosample Accession #: SAMN15040084

   
MLST: ST6(Pasteur)


Panel:  Vancomycin-Resistant Enterococci (VRE)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin 2S
Chloramphenicol >32R
Daptomycin 2S
Doxycycline 8I
High-Level Gentamicin 1>500SYN-R
High-Level Streptomycin 1>1000SYN-R
Levofloxacin 1S
Linezolid 2S
Penicillin 4S
Quinupristin/dalfopristin 216R
Rifampin 1S
Teicoplanin 0.5S
Vancomycin 2S
SDD (Susceptible Dose Dependent)
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 SYN-R: is not synergistic with cell-wall active agent(e.g., ampicillin, penicillin and vancomycin)
2 Reflects MIC of the combination
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-Ie, ant(6)-la, aph(3')-IIIa
Beta-lactam Z
Macrolide-Lincosamide-Streptogramin erm(B), Isa(A), mph(D)
Phenicols/Bicyclomycins catA7
Streptothricin sat4
Tetracyclines tet(M)
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
02/29/24Gene was added to Streptothricin : 'sat4'
02/29/24Gene was added to Tetracyclines: 'tet(M)'
02/29/24Gene was added to Phenicols/Bicyclomycins: 'catA7'
02/29/24Gene was added to Macrolide-Lincosamide-Streptogramin: 'mph(D)'
02/29/24Gene was added to Macrolide-Lincosamide-Streptogramin: 'Isa(A)'
02/29/24Category was added: Streptothricin
02/29/24Category was added: Tetracyclines
02/29/24Category was added: Phenicols/Bicyclomycins
02/29/24MLST was updated: from '---' to 'ST6(Pasteur)'
02/29/24Gene was added to Macrolide-Lincosamide-Streptogramin: 'erm(B)'
02/29/24Gene was added to Aminoglycoside: 'aph(3')-IIIa'
02/29/24Gene was added to Aminoglycoside: 'ant(6)-la'
02/29/24Gene was added to Aminoglycoside: 'aac(6')-Ie'
02/29/24Gene was added to Beta-lactam: 'Z'
02/29/24Category was added: Macrolide-Lincosamide-Streptogramin
02/29/24Category was added: Aminoglycoside
02/29/24Category was added: Beta-lactam
11/20/23Sequence Accession # changed: from '---' to 'SAMN15040084'
02/14/23INT was updated with a new value for Rifampin: from 'I' to 'S'
02/14/23MIC was updated with a new value for Rifampin: from '2' to '1'
11/20/18New Isolate added to panel
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