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Panel:   Tedizolid/Linezolid (AR-0781 - 0795) and VRE (AR-0474 - 0488) (Custom)
AR Bank # 0792 Enterococcus faecium
Study ID: VRE-12

Biosample Accession #: SAMN15040091

   
MLST: ST182(Pasteur)


Panel:  Vancomycin-Resistant Enterococci (VRE)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin 64R
Chloramphenicol 4S
Daptomycin 4SDD
Doxycycline <=1S
High-Level Gentamicin 1>500SYN-R
High-Level Streptomycin 2<=1000SYN-S
Levofloxacin >8R
Linezolid 1S
Penicillin >32R
Quinupristin/dalfopristin 30.5S
Rifampin 4R
Teicoplanin 1S
Vancomycin >64R
SDD (Susceptible Dose Dependent)
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 SYN-R: is not synergistic with cell-wall active agent(e.g., ampicillin, penicillin and vancomycin)
2 SYN-S: is synergistic with cell-wall active agent (e.g., ampicillin, penicillin and vancomycin) if the cell-wall active agent is also susceptible
3 Reflects MIC of the combination
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-Ie, aacA-ENT1
Efflux pumps/Other eat(A)
Glycopeptides VanB
Tetracyclines tet(M)
Trimethoprim dfrG
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
11/01/23Sequence Accession # changed: from '---' to 'SAMN15040091'
08/22/23INT was updated with a new value for Quinupristin/dalfopristin: from '---' to 'S'
08/22/23MIC was updated with a new value for Quinupristin/dalfopristin: from '---' to '0.5'
08/22/23Drug was added: Quinupristin/dalfopristin
06/23/23Gene was added to 'VanB'
06/23/23Category was added: 'Glycopeptides'
06/06/23MLST was updated: from '---' to 'ST182(Pasteur)'
06/05/23Gene was added to 'eat(A)'
06/05/23Gene was added to 'dfrG'
06/05/23Gene was added to 'tet(M)'
06/05/23Gene was added to 'aacA-ENT1'
06/05/23Gene was added to 'aac(6')-Ie'
06/05/23Category was added: 'Efflux pumps/Other'
06/05/23Category was added: 'Trimethoprim'
06/05/23Category was added: 'Tetracyclines'
06/05/23Category was added: 'Aminoglycoside'
06/05/23Category was removed: from '---' to 'Glycopeptides'
06/02/23Drug was removed: 'Quinupristin/dalfopristin'
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