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Panel:   Tedizolid/Linezolid (AR-0781 - 0795) and VRE (AR-0474 - 0488) (Custom)
AR Bank # 0476 Staphylococcus aureus
Study ID: BOR-16

Biosample Accession #: SAMN07291569
MLST: ST72(Pasteur)

Panel:  Staphylococcus with Borderline Oxacillin Susceptibility (BORSA) or (BOR)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Cefoxitin 216R
Ceftaroline <=0.25S
Clindamycin 3<=0.25R
Daptomycin <=0.5S
Doxycycline <=1S
Erythromycin >8R
Gentamicin <=2S
Levofloxacin <=0.5S
Linezolid 2S
Mupirocin <=4---
Oxacillin 4R
Penicillin >2R
Rifampin <=0.5S
Tetracycline <=1S
Trimethoprim/sulfamethoxazole 1<=0.5S
Vancomycin <=0.5S
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
1 Reflects MIC of first component
2 Cefoxitin is tested as a surrogate for mecA-mediated oxacillin resistance
3 Inducible clindamycin resistance detected
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aph-STPH
Beta-lactam mecA, Z
Efflux pumps/Other DHA-1, LMRS, MEPA
Fosfomycin fosB
Macrolide-Lincosamide-Streptogramin erm(C)
Quinolone norA
Tetracyclines tet(38)
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
08/11/22MIC was updated with a new value for Tetracycline: from '1.0' to '1'
08/11/22MIC was updated with a new value for Doxycycline: from '1.0' to '1'
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