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Panel:   Difficult-to-Detect Staphylococcus aureus harboring mecA (DTD)
AR Bank # 0978 Staphylococcus aureus
Study ID: DTD-02

Biosample Accession #: SAMN13412171

   

Disk Diffusion FOX: 12 mm, R*

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Difficult-to-Detect Staphylococcus aureus harboring mecA (DTD)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Cefoxitin 2 616R
Ceftaroline 0.5S
Clindamycin <=0.25S
Daptomycin <=0.5S
Doxycycline <=1.0S
Erythromycin 0.5S
Gentamicin <=2S
Levofloxacin 16R
Linezolid 2S
Mupirocin <=4---
Oxacillin 616R
Penicillin 6>2R
Rifampin <=0.5S
Tetracycline <=1.0S
Trimethoprim/sulfamethoxazole 1<=0.5S
Vancomycin 1S
S – I –R Interpretation (INT) derived from CLSI 2019 M100 S29
*Interpretation based on the individual drug MIC not considering other drug results, supplemental phenotypic tests, or genomic data
1 Reflects MIC of first component
2 Cefoxitin is tested as a surrogate for mecA-mediated oxacillin resistance
6 Interpretation based on the individual drug MIC not considering other drug results, supplemental phenotypic tests, or genomic data
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Beta-lactam mecA
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
07/08/19New Isolate added to panel
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