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Panel:   Drug Resistant Candida species (CAN)
AR Bank # 0315 Candida glabrata
Study ID: CAN-02

Biosample Accession #: Biosample Accession data is not yet available.

   
Location: USA
Source: blood

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antifungal agent for an isolate may commonly be ± 2 log2 (doubling dilution) different than what is posted on the CDC & FDA AR Isolate Bank website because this is the normal technical variability of antifungal susceptibility testing (for details, see M60 CLSI M27-ED4:2017 Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts). 

Panel:  Drug Resistant Candida species (CAN)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amphotericin B 10.38---
Anidulafungin 2R
Caspofungin 16R
Fluconazole 4SDD
Flucytosine >256---
Ibrexafungerp 0.25---
Itraconazole 1---
Micafungin 4R
Posaconazole 1---
Voriconazole 0.25---
1 Measured using Etest
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Echinocandins FKS1 R631G, FKS2 S663P
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Sabouraud Dextrose agar (SDA)

GROWTH CONDITIONS
Temperature: 30°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to SDA

Use streak plate method to isolate single colonies

Incubate inverted plate at 30°C for 48 hrs.

Note:  Candida  spp. stocks are stable at room temperature for several years.

Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
11/12/24Drug was added: 'Ibrexafungerp'
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