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Panel:   Acinetobacter baumannii (ACI)
AR Bank # 1267 Acinetobacter baumannii complex
Study ID: ACI-42

Biosample Accession #: SAMN35995984

   

Collection Year: 2017
Location: USA
Source: Sputum
MLST: ST2255(Pasteur), ST2838(Oxford)

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Acinetobacter baumannii (ACI)  |  Acinetobacter Add-ons (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin <=1S
Ampicillin/sulbactam 12S
Cefepime 1S
Ceftazidime 2S
Ceftriaxone 8S
Ciprofloxacin <=0.25S
Colistin 41I
Gentamicin 0.5S
Imipenem <=0.5S
Imipenem+chelators 2<=0.25---
Levofloxacin <=0.12S
Meropenem 0.25S
Minocycline <=4S
Piperacillin/tazobactam 1<=4S
Tigecycline 3<=0.5---
Tobramycin <=0.5S
Trimethoprim/sulfamethoxazole 1<=0.5S
MICs obtained by broth microdilution. Modal MIC is reported
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
1 Reflects MIC of first component
2 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
3 Based on FDA break points
4 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
 
Disclaimer:
AR gene prediction was performed using ARG-ANNOT, ResFinder and NCBI (ResGANNCBI) databases accessed on 2022-09-16. AR drug classes are assigned according to these databases. This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of used database or other antimicrobial resistance gene databases. For resistance determinant detection, 99-100% sequence identity and 100% sequence coverage from GAMMA and SRST2 was used. GAMMA uses amino acid sequence to assign gene alleles from assemblies; SRST2 uses nucleotide sequence to assign gene alleles from sequencing reads. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired. *Presence of Efflux Pump may not be associated with resistance. *MLST Type (and scheme), as determined by Torsten Seemann's MLST program. For Enterobacterales, the Pasteur MLST schemes are used except for E. coli for which both the Pasteur and Achtman schemes are reported. Unknown=novel MLST identified or allele unknown. For additional gene information please visit the Reference Gene Catalog - Pathogen Detection - NCBI (https://www.ncbi.nlm.nih.gov/pathogens/refgene), a non-redundant database of bacterial genes related to antimicrobial resistance, biocide and stress resistance, general efflux, virulence, or antigenicity.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2 °C for 20-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
06/27/23Sequence Accession # changed: from '---' to 'SAMN35995984'
05/05/23Isolate Status changed: from 'Pending' to 'Active'
05/01/23MIC was updated with a new value for Imipenem+chelators: from '---' to '<=0.25'
05/01/23Drug was added: Imipenem+chelators
04/25/23MLST was updated: from '---' to 'ST2838(Oxford)'
04/25/23MLST was updated: from '---' to 'ST2255(Pasteur)'
04/25/23Source was changed: from '---' to 'Sputum'
04/24/23Drug was removed: 'Imipenem/relebactam'
04/24/23Drug was removed: 'Imipenem+chelators'
04/24/23Drug was removed: 'Cefotaxime'
04/24/23INT was updated with a new value for Colistin: from '---' to 'I'
04/24/23MIC was updated with a new value for Tigecycline: from '<=2' to '<=0.5'
04/24/23MIC was updated with a new value for Colistin: from '---' to '1'
04/24/23MIC was updated with a new value for Ampicillin/sulbactam: from '<=1' to '2'
04/24/23Drug was added: Colistin
02/24/23INT was updated with a new value for Imipenem: from '---' to 'S'
02/08/23Isolate was removed from panel
02/08/23Isolate Status changed: from 'Active' to 'Pending'
01/11/23Isolate Status changed: from 'Pending' to 'Active'
12/27/22INT was updated with a new value for Trimethoprim/sulfamethoxazole: from '---' to 'S'
12/27/22INT was updated with a new value for Tobramycin: from '---' to 'S'
12/27/22INT was updated with a new value for Tigecycline: from '---' to 'S'
12/27/22INT was updated with a new value for Piperacillin/tazobactam: from '---' to 'S'
12/27/22INT was updated with a new value for Minocycline: from '---' to 'S'
12/27/22INT was updated with a new value for Meropenem: from '---' to 'S'
12/27/22INT was updated with a new value for Levofloxacin: from '---' to 'S'
12/27/22MIC was updated with a new value for Trimethoprim/sulfamethoxazole: from '---' to '<=0.5'
12/27/22MIC was updated with a new value for Tobramycin: from '---' to '<=0.5'
12/27/22MIC was updated with a new value for Tigecycline: from '---' to '<=2'
12/27/22MIC was updated with a new value for Piperacillin/tazobactam: from '---' to '<=4'
12/27/22MIC was updated with a new value for Minocycline: from '---' to '<=4'
12/27/22MIC was updated with a new value for Meropenem: from '---' to '0.25'
12/27/22MIC was updated with a new value for Levofloxacin: from '---' to '<=0.12'
12/27/22Drug was added: Trimethoprim/sulfamethoxazole
12/27/22Drug was added: Tobramycin
12/27/22Drug was added: Tigecycline
12/27/22Drug was added: Piperacillin/tazobactam
12/27/22Drug was added: Minocycline
12/27/22Drug was added: Meropenem
12/27/22Drug was added: Levofloxacin
12/27/22INT was updated with a new value for Imipenem/relebactam: from '---' to 'S'
12/27/22INT was updated with a new value for Imipenem+chelators: from '---' to '---'
12/27/22INT was updated with a new value for Imipenem: from '---' to '---'
12/27/22INT was updated with a new value for Gentamicin: from '---' to 'S'
12/27/22INT was updated with a new value for Ciprofloxacin: from '---' to 'S'
12/27/22INT was updated with a new value for Ceftriaxone: from '---' to 'S'
12/27/22INT was updated with a new value for Ceftazidime: from '---' to 'S'
12/27/22INT was updated with a new value for Cefotaxime: from '---' to 'S'
12/27/22INT was updated with a new value for Cefepime: from '---' to 'S'
12/27/22MIC was updated with a new value for Imipenem+chelators: from '---' to '<=0.25'
12/27/22MIC was updated with a new value for Imipenem: from '---' to '<=0.5'
12/27/22MIC was updated with a new value for Imipenem/relebactam: from '---' to '<=0.25'
12/27/22MIC was updated with a new value for Gentamicin: from '---' to '0.5'
12/27/22MIC was updated with a new value for Ciprofloxacin: from '---' to '<=0.25'
12/27/22MIC was updated with a new value for Ceftriaxone: from '---' to '8'
12/27/22MIC was updated with a new value for Ceftazidime: from '---' to '2'
12/27/22MIC was updated with a new value for Cefotaxime: from '---' to '4'
12/27/22MIC was updated with a new value for Cefepime: from '---' to '1'
12/27/22MIC was updated with a new value for Ampicillin/sulbactam: from '---' to '<=1'
12/27/22INT was updated with a new value for Ampicillin/sulbactam: from '---' to 'S'
12/27/22Drug was added: Imipenem/relebactam
12/27/22Drug was added: Imipenem+chelators
12/27/22Drug was added: Imipenem
12/27/22Drug was added: Gentamicin
12/27/22Drug was added: Ciprofloxacin
12/27/22Drug was added: Ceftriaxone
12/27/22Drug was added: Ceftazidime
12/27/22Drug was added: Cefotaxime
12/27/22Drug was added: Cefepime
12/27/22MIC was updated with a new value for Imipenem+chelators: from '---' to '<=0.25'
12/27/22MIC was updated with a new value for Imipenem: from '---' to '<=0.5'
12/27/22MIC was updated with a new value for Imipenem/relebactam: from '---' to '<=0.25'
12/27/22MIC was updated with a new value for Gentamicin: from '---' to '0.5'
12/27/22MIC was updated with a new value for Ciprofloxacin: from '---' to '<=0.25'
12/27/22MIC was updated with a new value for Ceftriaxone: from '---' to '8'
12/27/22MIC was updated with a new value for Ceftazidime: from '---' to '2'
12/27/22MIC was updated with a new value for Cefotaxime: from '---' to '4'
12/27/22MIC was updated with a new value for Cefepime: from '---' to '1'
12/27/22MIC was updated with a new value for Ampicillin/sulbactam: from '---' to '<=1'
12/27/22Drug was added: Ampicillin/sulbactam
12/27/22Location was changed: from '---' to 'USA'
12/27/22Collection Year was changed: from '---' to '2017'
12/27/22INT was updated with a new value for Amikacin: from '---' to 'S'
12/27/22MIC was updated with a new value for Amikacin: from '---' to '<=1'
12/27/22Drug was added: Amikacin
12/27/22Isolate Created
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