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Panel:   AR-1332, 1333, 1334 (Custom)
AR Bank # 1334 Pseudomonas aeruginosa
Study ID: IMP-12

Biosample Accession #: Biosample Accession data is not yet available.

mCIM Result: Positive    

Collection Year: 2022
Location: USA
Source: P-trap water
MLST: ST235(Pasteur)


Panel:  IMP-Type Metallo-ß-Lactamase (IMP)  |  AR-1332, 1333, 1334 (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin <=1S
Aztreonam 32R
Cefepime >32R
Cefepime/zidebactam 24---
Cefiderocol 0.5S
Ceftazidime >128R
Ceftazidime/avibactam 1>16R
Ceftolozane/tazobactam 1>16R
Delafloxacin >4R
Imipenem 32R
Imipenem/relebactam 132R
Imipenem+chelators 216---
Levofloxacin >8R
Meropenem >8R
Piperacillin/tazobactam 1128R
Tobramycin 8R
S – I –R Interpretation (INT) derived from CLSI 2026 M100 S36
SDD (Susceptible Dose Dependent)
1 Reflects MIC of first component
2 Cefepime to zidebactam ratio (1:1)
2 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-IIa, aacA4, aadA5, aph(3")-Ib, aph(3')-IIb, aph(6)-Id
Beta-lactam IMP-84, OXA-2, OXA-4, OXA-488, PDC-35
Efflux pumps/Other mexA, mexE, qacEdelta1
Fosfomycin fosA
Macrolide-Lincosamide-Streptogramin ere(A), mph(E), msr(E)
Phenicols/Bicyclomycins bcr1
Sulfonamides sul1
Trimethoprim dfrA5
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP); Brain Heart Infusion Agar with 5% Rabbit Blood (BHIA)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Ambient

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
04/10/26Category was added: Efflux pumps/Other
04/10/26Category was added: Macrolide-Lincosamide-Streptogramin
04/10/26Category was added: Aminoglycoside
04/10/26Category was added: Beta-lactam
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