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Panel:   PSA custom (Custom)
AR Bank # 0231 Pseudomonas aeruginosa
Study ID: PSA-03

Biosample Accession #: SAMN04901621

mCIM Result: Positive    
Source: Rectal Swab
MLST: 282(Pasteur)


Panel:  Pseudomonas aeruginosa (PSA)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin 8S
Aztreonam >64R
Cefepime >32R
Ceftazidime >64R
Ceftazidime/avibactam 38S
Ceftolozane/tazobactam 3>16R
Ciprofloxacin >8R
Colistin 22I
Doripenem >8R
Imipenem >64R
Imipenem+chelators 1>32---
Levofloxacin >8R
Meropenem >8R
Piperacillin/tazobactam 3128R
Tobramycin >16R
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
2 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
3 Reflects MIC of first component
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-IIc, aph(3')-IIb
Beta-lactam KPC-5, OXA-2, OXA-50, PDC-103
Efflux pumps/Other mexA, mexE, mexX
Fosfomycin fosA
Phenicols/Bicyclomycins bcr1
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
06/02/23Drug was removed: 'Gentamicin'
05/31/23Gene was added to 'mexX'
05/31/23Gene was added to 'mexE'
05/31/23Gene was added to 'mexA'
05/31/23Gene was added to 'bcr1'
05/31/23Gene was added to 'fosA'
05/31/23Gene was added to 'PDC-103'
05/31/23Gene was added to 'aph(3')-IIb'
05/31/23Category was added: 'Efflux pumps/Other'
05/31/23Category was added: 'Phenicols/Bicyclomycins'
05/31/23Category was added: 'Fosfomycin'
05/31/23Gene was removed from 'from '---' to ' PAO''
02/13/23MLST was updated: from '---' to '282(Pasteur)'
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