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Panel:   Custom D-406-24 (Custom)
AR Bank # 0804 Enterococcus faecalis
Study ID: VRE-24

Biosample Accession #: SAMN15040103

   
MLST: ST6(Pasteur)


Panel:  Vancomycin-Resistant Enterococci (VRE)  |  Custom D-406-24 (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin 1S
Chloramphenicol >32R
Daptomycin 1S
Doxycycline 16R
High-Level Gentamicin 2>500SYN-R
High-Level Streptomycin 2>1000SYN-R
Levofloxacin 1S
Linezolid 2S
Penicillin 4S
Quinupristin/dalfopristin 116R
Rifampin 2I
Tedizolid 0.25S
Teicoplanin 1S
Vancomycin 16I
SDD (Susceptible Dose Dependent)
S – I –R Interpretation (INT) derived from CLSI 2022 M100 S32
1 Reflects MIC of first component
2 SYN-R: is not synergistic with cell-wall active agent(e.g., ampicillin, penicillin and vancomycin)
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-Ie, ant(6)-Ia, aph(3')-IIIa
Beta-lactam Z
Glycopeptides VanB
Phenicols/Bicyclomycins catA7
Streptothricin sat4
Tetracyclines tet(L), tet(M)
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
11/01/23Sequence Accession # changed: from '---' to 'SAMN15040103'
06/23/23Gene was added to 'VanB'
06/23/23Category was added: 'Glycopeptides'
06/08/23Category was removed: from '---' to 'Glycopeptides'
06/06/23MLST was updated: from '---' to 'ST6(Pasteur)'
06/05/23Gene was added to 'sat4'
06/05/23Gene was added to 'tet(M)'
06/05/23Gene was added to 'tet(L)'
06/05/23Gene was added to 'catA7'
06/05/23Gene was added to 'aph(3')-IIIa'
06/05/23Gene was added to 'ant(6)-Ia'
06/05/23Gene was added to 'aac(6')-Ie'
06/05/23Gene was added to 'Z'
06/05/23Category was added: 'Streptothricin'
06/05/23Category was added: 'Tetracyclines'
06/05/23Category was added: 'Phenicols/Bicyclomycins'
06/05/23Category was added: 'Aminoglycoside'
06/05/23Category was added: 'Beta-lactam'
02/14/23Isolate Status changed: from 'Pending' to 'Active'
02/14/23Drug was removed: 'Omadacycline'
02/14/23Isolate was removed from panel
02/14/23Isolate Status changed: from 'Active' to 'Pending'
02/14/23INT was updated with a new value for Daptomycin: from 'SDD' to 'S'
02/14/23INT was updated with a new value for Omadacycline: from '---' to 'S'
02/14/23INT was updated with a new value for Tedizolid: from '---' to 'S'
02/14/23INT was updated with a new value for Daptomycin: from 'S' to 'SDD'
02/14/23MIC was updated with a new value for Teicoplanin: from '0.5' to '1'
02/14/23MIC was updated with a new value for Tedizolid: from '---' to '0.25'
02/14/23MIC was updated with a new value for Omadacycline: from '---' to '<=0.12'
02/14/23Drug was added: Omadacycline
02/14/23Drug was added: Tedizolid
11/20/18New Isolate added to panel
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