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Panel:   AR-0387, AR-0401-0426 (Custom)
AR Bank # 0418 Campylobacter coli
Study ID: GI-18

Biosample Accession #: SAMN12648968

   


Panel:  Enteric Pathogen Diversity (GI)  |  AR-0387, AR-0401-0426 (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Azithromycin >64NWT
Ciprofloxacin 0.12S
Erythromycin >64R
Florfenicol 1WT
Gentamicin 1WT
Nalidixic acid <=4WT
Tetracycline 0.25S
S – I –R Interpretation (INT) derived from M45 2016 S3
WT (wild type; =ECVs) – NWT (non-wild type; =ECVs) based on epidemiological cutoff values (ECVs) adopted from the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2021)
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Beta-lactam OXA-61
Macrolide-Lincosamide-Streptogramin 23S(M)
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP); Brain Heart Infusion Agar with 5% Rabbit Blood (BHIA)

GROWTH CONDITIONS
Temperature: 37°C
Atmosphere: Micro-aerobic

PROPAGATION PROCEDURE
Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
Open vial aseptically to avoid contamination
Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
Aseptically transfer the loop to BHIA
Use streak plate method to isolate single colonies
Incubate inverted plate at 37°C for 24-48 hrs., then subculture to BAP and allow 48 hours of incubation prior to performing Antimicrobial Susceptibility Testing (AST).
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
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