Skip directly to site content
Are You Still There?
Due to inactivity, you will be signed out in two minutes unless you click 'Continue'.

Are you still there?

Due to inactivity, you will be signed out in two minutes unless
you click 'Continue'.

Panel:   D-594-23 MTA (Custom)
AR Bank # 1107 Pseudomonas aeruginosa
Study ID: NEW-33

Biosample Accession #: SAMN31096620

mCIM Result: Positive    
MLST: 235(Pasteur)


Panel:  Isolates with New or Novel Antibiotic Resistance (NEW)  |  D-594-23 MTA (Custom)  |  PSA SD (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin 32I
Aztreonam 8S
Cefepime >32R
Cefepime/zidebactam 24---
Ceftazidime >128R
Ceftazidime/avibactam 1>16R
Ceftolozane/tazobactam 1>16R
Ciprofloxacin 8R
Colistin 31I
Imipenem 2S
Imipenem/relebactam 12S
Imipenem+chelators 41---
Levofloxacin >8R
Meropenem 8R
Tobramycin >16R
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
1 Reflects MIC of first component
2 Cefepime to zidebactam ratio (1:1)
3 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
4 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-Ib-G, aph(3')-IIb
Beta-lactam HMB-2, OXA-488, PDC-35, TEM-1
Efflux pumps/Other mexA, mexE, qacEdelta1
Fosfomycin fosA
Phenicols/Bicyclomycins bcr1
Quinolone crpP
Sulfonamides sul1
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
06/14/23Drug was removed: 'Gentamicin'
12/02/22Isolate Status changed: from 'Pending' to 'Active'
11/09/22Gene was added to 'QACEDELTA1'
11/09/22Category was removed: from '---' to 'Other'
11/08/22MLST was updated
11/08/22mCIM changed
11/08/22Gene was added
11/08/22Category was added
10/26/22Gene was added
10/26/22Category was added
10/26/22Gene was added
10/26/22Category was added
10/26/22Gene was added
10/26/22Category was added
10/26/22INT was updated with a new value for Tobramycin: from '---' to 'R'
10/26/22INT was updated with a new value for Meropenem: from '---' to 'R'
10/26/22INT was updated with a new value for Levofloxacin: from '---' to 'R'
10/26/22INT was updated with a new value for Imipenem+chelators: from '---' to '---'
10/26/22INT was updated with a new value for Imipenem: from '---' to 'S'
10/26/22INT was updated with a new value for Imipenem/relebactam: from '---' to 'S'
10/26/22INT was updated with a new value for Gentamicin: from '---' to 'R'
10/26/22INT was updated with a new value for Colistin: from '---' to 'I'
10/26/22INT was updated with a new value for Ciprofloxacin: from '---' to 'R'
10/26/22INT was updated with a new value for Ceftolozane/tazobactam: from '---' to 'R'
10/26/22INT was updated with a new value for Ceftazidime: from '---' to 'R'
10/26/22INT was updated with a new value for Ceftazidime/avibactam: from '---' to 'R'
10/26/22INT was updated with a new value for Cefepime: from '---' to 'R'
10/26/22INT was updated with a new value for Cefepime/zidebactam: from '---' to '---'
10/26/22INT was updated with a new value for Aztreonam: from '---' to 'S'
10/26/22INT was updated with a new value for Amikacin: from '---' to 'I'
10/26/22MIC was updated with a new value for Tobramycin: from '---' to '16'
10/26/22MIC was updated with a new value for Meropenem: from '---' to '8'
10/26/22MIC was updated with a new value for Levofloxacin: from '---' to '8'
10/26/22MIC was updated with a new value for Imipenem+chelators: from '---' to '1'
10/26/22MIC was updated with a new value for Imipenem: from '---' to '2'
10/26/22MIC was updated with a new value for Imipenem/relebactam: from '---' to '2'
10/26/22MIC was updated with a new value for Gentamicin: from '---' to '16'
10/26/22MIC was updated with a new value for Colistin: from '---' to '1'
10/26/22MIC was updated with a new value for Ciprofloxacin: from '---' to '8'
10/26/22MIC was updated with a new value for Ceftolozane/tazobactam: from '---' to '16'
10/26/22MIC was updated with a new value for Ceftazidime: from '---' to '128'
10/26/22MIC was updated with a new value for Ceftazidime/avibactam: from '---' to '16'
10/26/22MIC was updated with a new value for Cefepime: from '---' to '32'
10/26/22MIC was updated with a new value for Cefepime/zidebactam: from '---' to '4'
10/26/22MIC was updated with a new value for Aztreonam: from '---' to '8'
10/26/22MIC was updated with a new value for Amikacin: from '---' to '32'
10/26/22Drug was added: Tobramycin
10/26/22Drug was added: Meropenem
10/26/22Drug was added: Levofloxacin
10/26/22Drug was added: Imipenem/relebactam
10/26/22Drug was added: Imipenem+chelators
10/26/22Drug was added: Imipenem
10/26/22Drug was added: Gentamicin
10/26/22Drug was added: Colistin
10/26/22Drug was added: Ciprofloxacin
10/26/22Drug was added: Ceftolozane/tazobactam
10/26/22Drug was added: Cefepime/zidebactam
10/26/22Drug was added: Ceftazidime/avibactam
10/26/22Drug was added: Ceftazidime
10/26/22Drug was added: Cefepime
10/26/22Drug was added: Aztreonam
10/26/22Drug was added: Amikacin
09/30/22Sequence Accession # changed
11/18/20New Isolate added to panel
Top of Page
TOP