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Complement-fixation test. An antiserum for Eyach with a homologous titer of 32 was tested against antigens for 49 non-group B tick-borne viruses; the serum reacted only with Colorado tick fever (CTF) antigen, with a titer of 16. |
In another test, 15 polyvalent grouping sera or ascitic fluids were tested against an antigen for Eyach; only the fluid containing CTF antibodies reacted with Eyach. |
The CF relationship between CTF and Eyach is as follows: |
|
Antigen | Serum |
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| Eyach | CTF |
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| Serum titer/antigen titer | Eyach | 128/128 | 64/64 | CTF | 32/64 | 256/512 |
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Neutralization tests: In newborn mice, by intracerebral route ofinoculation. |
|
Serum | Virus |
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| Eyach | CTF |
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|
| a LNI expressed in dex | Eyach | 3.3 a | 0.3 | CTF | 2.9 | 4.0 | Normal | |
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