| | | In CF testing, a SM liver antigen did not react with the following ascitic fluids: EEE, WEE, VEE, Mayaro, Una, Pixuna, Ilheus, SLE, yellow fever, dengue 2, Murray Valley encephalitis, Cache Valley, Tensaw, Silverwater, Turlock, California (La Crosse and trivittatus), Oropouche, Sathuperi, Aruac, Ieri, Sororoca, Anopheles A, Anopheles B, VS-Indiana, CTF, Buttonwillow, Flanders, Herpes simplex, Broad Group A, Broad Group B, Broad California, Broad Guama, Broad Capim, Broad Bunyamwera and Broad Simbu. A hyperimmune ascitic fluid of the prototype failed to inhibit hemagglutinins of EEE, WEE, SLE, MVE, LAC, Tensaw and Buttonwillow. | | | | | Gumbo Limbo Antigen a | Gumbo Limbo Mouse Hyperimmune Ascitic Fluid |
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| | HI b | CF c | NT d | HI | CF | NT |
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| Immune Ascitic Fluid or Antigen | Ht/Ho | Ht/Ho | Ht/Ho | Ht/Ho | Ht/Ho | Ht/Ho |
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| | a Antigen prepared by acetone extraction of infectious mouse serum. | | b Titers expressed as reciprocal of highest serum dilution inhibiting 4-8 units of antigen. | | c Titers expressed as reciprocal of highest serum dilution fixing 5-50% units of complement in box CF. | | d Tissue culture neutralization index derived from plaque-reduction neutralization test in BHK-21 cells. Titers expressed as dex. | | Oriboca | <10/160 | 64/64 | 0.4/3.7 | 0/40 | 64/512 | 1.2/>4.7 | | Apeu | <10/320 | 64/64 | 0.2/4.4 | 20/40 | 64/512 | 1.9/>4.7 | | Maratuba | 10/640 | 128/128 | 3.2/4.1 | 0/40 | 64/512 | 2.0/>4.7 | | Caraparu | 10/80 | 32/32 | 0.1/3.7 | 10/40 | 32/512 | 2.1/>4.7 | | Itaqui | 10/160 | 32/64 | 2.5/4.2 | 0/40 | 32/512 | 2.0/>4.7 | | Restan | 20/1280 | 64/512 | 3.7/>5.5 | 0/40 | 64/512 | 3.1/>4.7 | | Ossa | <10/320 | 32/64 | 0.2/>5.3 | 20/40 | 32/512 | 0.9/>4.7 | | Nepuyo | | 256/512 | 3.4/3.1 | | 256/512 | 1.7/>4.7 | | Murutucu | <10/80 | 64/128 | 3.3/4.3 | 0/40 | 64/512 | 2.8/>4.7 | | Madrid | <10/40 | 32/128 | 0.0/3.4 | 0/40 | 32/512 | 0.5/4.7 | | Gumbo Limbo | 40 | 512 | >4.7 | 40 | 512 | >4.7 |
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