Physicochemical
RNA, Single Strand
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3 (29)
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L=2.7x106M(S)
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4
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Nucleoprotein (N): 45-50,000 MW; glycoproteins (G1,G2):
55-56,000 MW, 72,000 MW; (L) 200,000 MW (30)
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1.16-1.17; 1.20-1.21 in sucrose; CsCl2
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450 S (31)(S)
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1.19; 1.25 in sucrose; CsCl2
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(S)
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| Stability of Infectivity (effects) |
Maximum stability at pH 7.6; inactived at pH 3.0
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20%
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<1.0 dex
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6.0 dex
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5%
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<1.0 dex
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6.0 dex
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0.1%
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<1.0 dex
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6.0 dex
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Inactivated with 70% ethanol or 0.5% iodine; cobalt irradiation
at 1.3x107 rads
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| Virion Morphology |
Bunyavirus-like (24-28)
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80-115 nm
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nm
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nm
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Electron microscopy
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Surface projections, 11-13 nm; lipid envelope
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| Morphogenesis |
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Cisternae of endoplasmic reticulum (28)
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Seen with immunoperoxidase staining (28)
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| Hemagglutination |
Yes
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Apodemus lung tissue or A-549 cell cultures
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Gander***
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5.8-7.2
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5.8-6.4
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4dC, 25dC, 37dC
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25dC
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HA obtained with SMB extracted by sucrose-acetone and reacted with gooseRBC's(32)
* Proposed genus designation** If the virus is handled in rodents, then Level 4.
***In addition to goose, Human O, rhesus monkey, guinea pig RBC's used
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NT, IFA, immune adherence HA, HI
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HA obtained with SMB extracted by sucrose-acetone and reacted with gooseRBC's(32)
* Proposed genus designation** If the virus is handled in rodents, then Level 4.
***In addition to goose, Human O, rhesus monkey, guinea pig RBC's used
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