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| Screening HI tests indicated antigenic relationship of Highlands J virus with group A arboviruses, with closest relationship to members of the WEE complex [1] , [3] . The data below show the relationship of Highlands J virus to members of the WEE complex by HI, CF, and serum dilution plaque-reduction neutralization tests (PRNT). |
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| Results of CF Tests |
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| | Antibody to: |
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| Viruses or Antigens | WEE | HI | Y62-33 | SIN | WHA | AURA |
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| | a Serum titer/antigen titer; 0 = <8/<8. | | WEE | 128/128 a | 32/32 | 256/>512 | 16/32 | 0 | 0 | | HJ | 0 | 32/8 | 64/32 | 0 | 0 | 0 | | Y62-33 | 128/32 | 32/8 | 256/128 | 0 | 0 | 0 | | SIN | 16/8 | 0 | 128/8 | 64/8 | 64/32 | 0 | | WHA | 0 | 0 | 0 | 0 | 64/32 | 0 | | AURA | 16/32 | 0 | 0 | 0 | 0 | 32/128 |
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| Results of HI Tests |
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| | HI titer of antibody to: |
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| Viruses or Antigens | WEE | HI | Y62-33 | SIN | WHA | AURA |
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|
| | b 0 = <10. | | WEE | 2560 | 80 | 320 | 40 | 10 | 0 b | | HJ | 1280 | 320 | 160 | 80 | 20 | 0 | | Y62-33 | 2560 | 80 | 1280 | 80 | 20 | 0 | | SIN | >5120 | 320 | 320 | 1280 | 320 | 0 | | WHA | 1280 | 20 | 80 | 160 | 320 | 0 | | AURA | 320 | 10 | 10 | 10 | 10 | 80 |
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| RESULTS OF PRNT [4] |
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| 50% PRNT titer of antibody to: |
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| Viruses | WEE | HJ | Y62-33 | SIN | WHA | AURA |
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| | 0 = <10 | | WEE | 700 | 70 | 80 | 0 | 0 | 0 | | HJ | 440 | 2400 | 230 | 140 | 10 | 0 | | Y62-33 | 50 | 0 | 49000 | 0 | 0 | 0 | | SIN | 150 | 0 | 0 | 770 | 65 | 0 | | WHA | 120 | 0 | 0 | 50 | 100 | 0 | | AURA | 0 | 0 | 0 | 0 | 0 | 30 |
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| Although Highlands J virus was readily distinguished from WEE strains by various serological tests initially, it was considered to be an antigenic variant of WEE [5] , [6] . |
| Recently, kinetic HI and neutralization tests have distinguished HJ virus from strains of WEE as well as other members of the WEE complex, including Fort Morgan virus and other alphaviruses [7] . Highlands J virus was indistinguishable from WEE-like viruses isolated in the eastern United States. Accordingly, it was specified that HJ and Fort Morgan viruses be accorded separate virus status in the WEE complex [7] . RNA oligonucleotide fingerprints and subunit antisera also distinguish HJ virus from strains of WEE and other alphaviruses [3] . In reevaluating antigenic relationships of alphaviruses, SIRACA has determined that Highlands J virus is a separate virus in the WEE complex [8] . |
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