| The virus antigen (mouse brain and liver) did not react in CF testing with the following ascitic fluids: groups A, B, C, Guama, Capim, Bunyamwera; and specific ascitic fluids to Piry, Itaporanga, Jurona, Pacui, and Irituia viruses. Liver antigen gave a reaction of 4/>8 with a grouping ascitic fluid for the Phlebotomus fever group. In a second CF test, this liver antigen was negative with ascitic fluids of viruses of the Phlebotomus fever group: Icoaraci, Bujaru, Anhanga, Sicilian, Chagres, and Urucuri, but reacted with the ascitic fluid for Candiru virus. | | Ascitic fluids |
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Antigens | BeAn 213452 | Candiru | Control |
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| a Serum titer/antigen titer; 0 = <4/<4 | BeAn 213452 | 128/1024 a | 64/256 | 0 | Candiru | 128/128 | 128/256 | 0 | Control | 0 | 0 | 0 |
| A cross NT with Candiru gave the following results: | | Virus |
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Ascitic fluid | BeAn 213452 | Candiru |
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| b LNI in dex. | BeAn 213452 | > 5.8 b | 0.6 | Candiru | 0.7 | 3.4 | Control | <1.0 | 0 |
| At YARU, it was whown by CF testing that the registered virus is not related to Naples, Sicilian, Ib 15736, Chagres, Punta Toro, and Karimabad viruses. A relationship to Candiru and Nique viruses was demonstrated. | Immune serum to BeAn 213452 (titer of >256) did not react in CF test with the antigens of Anhanga, Bujaru, Chagres, Karimabad, Sicilian, Naples, Arumowot, Icoaraci, Punta Toro, and Gabek Forest. It reacted at 16/8 with Buenaventura and 16/>256 with Nique antigen. | Plaque reduction neutralization tests performed at the National Institute of Health, Hawaii [1] revealed that BeAn 213452 was not neutralized by a 1:10 dilution of the immune sera to the following Phlebotomus fever viruses: Alenquer (BeH 301101), St. Floris, Gordil, Naples, Sicilian, Arumowot, Gabek Forest, Salehabad, Karimabad, Punta Toro, Chagres, Itaporanga, Icoaraci, Candiru, Pacui, Anhanga, Urucuri, Buenaventura, Frijoles, Aguacate, Cacao, Nique, Caimito, Chilibre, Rio Grande, Charleville. | |
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