Physicochemical
|
|
|
|
(S)
|
|
|
|
|
|
|
|
|
(S)
|
|
(S)
|
|
Stability of Infectivity (effects) |
|
1:2
|
5.5 dex
|
8.0 dex
|
|
|
|
0.1%
|
<5.0 dex
|
8.0 dex
|
Trypsin 0.05% After treatment: <2.7 dex Control titer: 8.0 dex
|
|
Virion Morphology |
Spherical particles
|
About 33 nm; 35-42 nm
|
nm
|
nm
|
Electron microscopy (negative stain; sections(8))
|
|
|
|
Morphogenesis |
|
|
|
|
|
|
Hemagglutination |
Yes
|
SMB ext. by sucrose-acetone
|
Goose
|
5.9-7.0
|
6.5-6.7
|
4dC-37dC
|
4dC
|
Also used centrifugation, protamine precipitation and calcium
phosphate chromatography to purify HA (2,3)
* Rattus mulleri validus, R.sabanus vociferans.
|
HI, CF, NT, agar gel precipitation (7)
|
Also used centrifugation, protamine precipitation and calcium
phosphate chromatography to purify HA (2,3)
* Rattus mulleri validus, R.sabanus vociferans.
|
|