| | | Neutralization tests using 2-3 day mice inoculated intraperitoneally with Lone Star virus and specific mouse immune ascitic fluids prepared against 17 arboviruses from the United States and Canada gave negative results. The homologous (Lone Star virus) immune ascitic fluid neutralized more than 5 dex of virus. Negative results were with the following: Group A (eastern and western encephalitis); Group B (St. Louis encephalitis, Powassan, Modoc, Rio Bravo); Bunyamwera Group (Cache Valley and Tensaw); California (California encephalitis and trivittatus); Simbu (Mermet (AV137); Hughes (Hughes); Turlock (Turlock); Ungrouped (Colorado tick fever, Hart Park, Flanders, Silverwater and Sawgrass). | | Complement Fixation | | First series: The titer of antigen in homologous system was greater than 128 and the titer of immune ascitic fluid was 16 to 32. Negative results with 17 agents listed above for the NT as well as the following: Venezuelan equine encephalitis, Mahogany Hammock, Shark River, Gumbo Limbo, Tamiami, Cowbone Ridge, Montana Myotis leukoencephalitis and EMC. | | Second series done by Dr. Jordi Casals, WHO International Arbovirus Reference Center, Yale Arbovirus Research Unit. Lone Star antiserumm reacted only with its own antigen, in a titer equal to or greater than 256. In these tests Lone Star immune serum was tested in two-fold dilutions ranging from 1:8 to 1:256 against the following tick virus antigens diluted 1:4, 1:8 and 1:16. Negative results were obtained with the following: Bakau, Bandia, Bhanja, Chenuda, Colorado tick fever, DGK, Dugbe, Farallon, Ganjam, Grand Arbaud, Hazara, Johnston Atoll, Kaisodi, Kemerovo, Lanjan, Lipovnik, Mamawa, Matucare, Nyamanini, Qalyub, Quaranfil, Sawgrass, Silverwater, Soldado, Tribec, Upolu, Uukuniemi, Wanowrie, Hughes, Tick 39 (Thailand). | | |
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