Blood (M)(LV), CNS (LV), liver (M), spleen (M), nasopharyngeal (M), salivary gland (M) (from saliva swab)
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Newborn mice and suckling hamsters; WI-26 human diploid embryo fibroblast
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| | | | | Cell system (a) | Virus passage history (b) | Evidence of Infection | | |
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| | CPE | PLAQUES | Growth Without CPE | | |
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| | Day (c) | Extent (d) | Titer TCD50/ml (e) | Day (c) | Size (f) | Titer PFU/ml (e) | +/- (g) | | |
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| | Marburg virus infects and replicates in a variety of mammalian cell cultures, including primary and continuous cell cultures, primary and continuous primate cell cultures, primary and continuous guinea pig cell cultures and BHK-21 cell cultures. In most instances, the development of CPE was variable or absent. One primary and one continuous primate cell culture, a continuous human cell culture and BHK-21 exhibited distinct CPE after infection with Marburg virus. Variable CPE was produced in Vero cell cultures, and CPE endpoints generally were 10-100 times lower than infective virus titrations. See Reference 14. | | |
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