| | | The viral antigen did not react in CF tests with 20 mouse ascitic fluids made from the following argasid tick viruses: Farallon (AR846), Hughes (Orig.), Punta Salinas (AR888), Raza (5/8/64), Sapphire II (strain 14), Soldado (6446), Zirqa (POR7866), all of the Huges serogroup; Baku (Liev 46A), Chenuda(AR1170), Huacho (AR883), Sixgun City (strain 10), all of the Kemerovo serogroup; Midway (RML47153), Nyamanini (AR1304) of the Nyamanini serogroup; Johnston Atoll (RML39579), Quaranfil (AR1113), of the Quaranfil serogroup; Bandia (AP/A611), Qalyub (AR370), of the Qalyub serogroup; and Chobar Gorge (701700-8), Matucare (21343), both serologically ungrouped. | | Related by CF test to Upolu virus, but clearly distinguishable by plaque reduction NT and indirect immunofluorescence tests, as follows: | | | | Hyperimmune Mouse Ascitic Fluid |
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| Complement-fixation a | | |
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| | Test 1 | Test 2 | Indirect immuno-fluorescence b | Virus-detection neutralization c |
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| Virus or Antigen | 65660-8 | C5581 | 65660-8 | C5581 | 65660-8 | C5581 | Control | NI 65660-8 | NI C5881 |
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| | a Reciprocal of serum titer at optimal dilution of antigen; lowest dilution tested = 1:4. | | b Reciprocal of highest serum dilution giving a positive reaction. | | c Control: Virus titer, in Vero cell plaque-forming units/ml, after incubation with normal serum. NI: Neutralization index (dex reduction of Vero cell plaque titer after incubation of virus/antiserum mixture). | | Aransas Bay | | | | | | | | | | (65660-8) | 128 | 16 | | | 64 | 8 | 8.1 | >6.1 | 0.1 | | (65660-42) | | | 32 | 4 | | | | | | | Upolu(C5581) | 64 | 64 | 32 | 32 | 8 | 256 | 8.2 | 2.0 | 4.8 |
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