| |
| Hemagglutation inhibition. |
| Sucrose-acetone extracted mouse and hamster brain antigens were prepared and tested for hemagglutination properties according to the method of Clarke and Casals with negative results. |
| Complement fixation. |
| The sucrose-acetone extracted mouse brain was used as commplement-fixing antigen. Hyperimmune serum prepared in mice was used. Cross complement- fixations were done against polyvalent immune sera of the major groups of arbovirus that were available at the Yale Reference Center. No reactions were observed. Also, no relationship was encountered when reagents from the following viruses isolated from ticks and bats were tested: |
| |
| Tick viruses | Bat viruses |
|---|
|
| | Farallon | Bhanja | Quaranfil | Bukalasa bat | | Chenuda | Wanowrie | Sawgrass | Dakar bat | | Colorado tick fever | Johnston Atoll | Silverwater | Entebbe bat | | Hughes | Kaisodi | Soldado | Kern Canyon | | Dugbe | Kemerovo | Thogoto | Lagos bat | | Ganjam | Lanjan | Tribec | MML | | Nyamanini | Manawa | Uukuniemi | Rio Bravo | | Wad Medani | | | |
|
| Neutralization. |
| Immune sera from Machupo, Junin, Tacaribe, and Amapari gave no demonstrable protection. |
| Indistinguishable complement-fixation reaction was found with another isolate from O. boliviensis ticks, MARU 21342. |
| |
| Complement-fixation Test | | Neutralization Test |
|---|
| | Antibody | | | Antibody |
|---|
| Antigen | Matucare | 21342 | | Virus | Matucare | 21342 |
|---|
|
| | a Serum titer/antigen titer | | b Neutralizing indices in dex | | Matucare | 128/64 a | 128/32 | | Matucare | 1.6 b | 1.3 | | MARU 21342 | 128/64 | 128/32 | | 21342 | 1.9 | 1.8 |
|
| |
