| | | HI test gives negative results with the following viruses: Chikungunya, O'nyong nyong, Sindbis, Semliki, Middelburg, Ntaya, Wesselsbron, Usutu, West Nile, Dakar bat, Uganda S, yellow fever, Zika, Bunyamwera. | | CF homologous titer = 32/32. | | CF screening: YM 31/65 antigen gives negative results with the following sera from mice: | | |
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| | * Homologous antiserum titre/antigen titre. The antiserum titres are the maxima. The antigen titres given are the highest at which the antiserum is maximal (i.e. optimal). | | ** Antiserum supplied by International Reference Center. | | Group A: | | Bwamba group: | |
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| Chikungunya | 128/10 * | Bwamba | 32/8 | | O'nyong nyong | 64/8 | Pongola | 32/8 | | Semliki Forest | | | | Sindbis | 64/32 | Group Simbu: | |
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| Middelburg | 32/8 | Simbu | 64/128 | | Ndumu | 64/8 | Ingwavuma | 128/128 | | Group B: | | Others: | |
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| Ntaya | 16/16 | Quaranfil | 64/8 | | Wesselsbron | 128/32 | Chenuda | 64/4 | | Usutu | 256/8 | Nyamanini | 128/64 | | West Nile | 32/32 | Wad Medani | ** | | Dakar bat | 32/8 | Thogoto | 16/8 | | Uganda S | 32/6 | Mossuril | 32/128 | | Yellow fever | 16/16 | Nyando | 16/64 | | Zika | 64/6 | Lumbo | 128/4 | | Spondweni | 64/16 | Bandia | 256/128 | | Bukalasa | 64/16 | Lagos bat | ** | | Entebbe Bat | 80/80 | Olifantsvlei | 40/40 | | | | Gossas | 64/16 | | Group Bunyamwera: | | |
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| Bunyamwera | 16/32 | Ungrouped: | |
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| Germiston | 128/8 | Lebombo | | Ilesha | 32/256 | Tanga | 16/32 | | Shokwe | 521/128 | Tataguine | 64/64 | | | | Witwatersrand | 256/256 | | | | YM 176 | 64/32 | | | | BA 40 | 32/64 | | | | IPD/A 318 | 256/128 | | | | IPD/A 763 | 16/8 |
| | Nkolbisson virus is related to Kern Canyon, Barur and the unregistered Fukuoka virus and they form the Kern Canyon antigenic group [5] . On basis of these tests, Nkolbisson is apparently an unrelated virus. | | |
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