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  Playas   PLAV

Possible Arbovirus

No

3

Isufficient experience with virus; i.e., experience factor from SALS surveys was less than 500 in laboratory facilities with low biocontainment.
Other Information
 

Bunyamwera
Section V - Antigenic Relationship and Lack of Relationship to Other Viruses
 
Antigen of strain 75V-3066 was tested by CF with immune mouse ascitic fluids (MIAF) representing viruses of the following serogroups: A, B, C, Bunyamwera, vesicular stomatitis, Capim, Turlock, Gamboa, Patois, Minatitlan, Guama, California, Simbu and Hart Park. The only reaction was with the Bunyamwera grouping fluid. Because more than 240 strains of Maguari virus were isolated from Aedes species (principally or exclusively scapularis) mosquitoes from essentially the same area as that from which strain 75V-3066 was isolated, strains 75V-3066, 75V-5758, and 75V-5938 (all from A. taeniorhynchus) were tested by neutralization with NIAF prepared against prototype Maguari and Ecuadorian topotype Maguari (strain E4-3484) viruses and with a Bunyamwera grouping MIAF, with the following results [1] :
 
Titer of N Antibody to:
Virus Strain BeAr 7272 E4-3484 Broad Group BUN


Maguari BeAr 7272 320 640 >=1280
Maguari E-43484 640 >=1280 >=1280
Playas 75V-3066 <40 40 160
Playas 75V-5758 <40 40 160
Playas 75V-5938 <40 40 160
Strain 75V-3066 virus and MIAF was tested with Bunyamwera serogroup viruses by serum dilution-plaque reduction neutralization (N) tests in Vero cells.
 
75V-3066 75V-3066
Virus or MIAF (Homologous) Virus MIAF Virus or MIAF (Homologous) Virus MIAF


b = <20
75V-3066 (160) 160 160 Taiassui (320) - -
Maguari (320) 40 - Tensaw (640) 40 -
Cache Valley (160) 40 - Tlacotalpan (320) 20 -
Guaroa (160) 40 - Tucunduba (160) - -
Anhembi (640) 20 - Wyeomyia (640) 20 -
Kairi (320) - b - Batai (>=2560) 160 -
Lokern (2560) 40 - Beliefe (2560) 20 -
Maine Drain (1280) - - Birao (>=2560) 80 -
Northway (160) - - Bunyamwera (>=1280) 20 -
Sororoca (>=2560) 20 - Germiston (2560) - -
Santa Rosa (1280) - - Shokwe (640) 20 -
Ilesha (>=5120) 160 -
 
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