Arbovirus Catalog

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Physicochemical
Pieces (number of genome segments)	Infectivity	Sedimentation Coefficients(s) (S)
Percentage wt, of Virion Protein	Lipid	Carbohydrate
Virion Polypeptides: Number	Details
Non-virion Polypeptides: Number	Details
Virion Density	Sedimentation Coefficients(s) (S)
Nucleocapsid Density	Sedimentation Coefficients(s) (S)

Stability of Infectivity (effects)
pH (infective range)
Lipid Solvent (ether - % used to test)	After Treatment Titer	Control Titer
Lipid Solvent (chloroform)	After Treatment Titer	Control Titer
Lipid Solvent (deoxycholate) 1:1000	After Treatment Titer nil	Control Titer 5.0 dex
Other (formalin, radiation)

Virion Morphology
Shape Icosahedron	Dimensions
Mean nm	Range 40-45nm
Measurement Method EM of thin sections and phosphotungstic preps	Surface Projections/Envelope Enveloped	Nucleocapsid Dimensions, Symmetry 39 nm, cubic symmetry

Morphogenesis
Site of Constituent Formation in Cell Cytoplasm	Site of Virion Assembly Cytoplasmic vacuoles	Site of Virion Accumulation Cytoplasm
Inclusion Bodies None	Other

Hemagglutination
Hemaggiutination Yes	Antigen Source SMB ext. by sucrose-acetone; acetone-ether; borate + prot.	Erythrocytes (species used) Goose
pH Range 6.4-6.8	pH Optimum 6.6
Temperature Range 4-37dC	Temperature Optimum 22dC
Remarks
Serologic Methods Recommended HI, CF, NT
Footnotes