| Complement-fixation test. Grouping immune sera or ascitic fluids for the following listed groups, reacted negatively against Punta Salinas antigen. The sera were in dilutions from 1:4 and up and the antigen in dilution 1:8. The grouping reagents used were: Group A, B, C, Anopheles A, Bunyamwera, Bwamba, California, Capim, Guama, Nyando, Palyam, rhabdoviruses, Simbu, Tacaribe, Tete, and VSV. | The following type specific immune sera or ascitic fluids, in dilutions from 1:4 and up, have reacted negatively with antigen Punta Salinas, in dilution 1:8: Aruac, Changuinola, Cotia, Kern Canyon, LCM, Lukuni, Nariva, Pacui, rabies and Tembe. | In cross complement-fixation tests with about 40 distinct non-group B tick- borne viruses, Punta Salinas serum and antigen reacted only with members of the Hughes group. An example of the relationship is shown in the table: | | Complement-Fixation Test |
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Antigen | Serum |
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| Punta Salinas | Soldado |
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| Serum titers against 8 and 16 units of antigen. | Hughes | 32 | 8 | Farallon | 32 | 8 | Soldado | 8 | 128 | Punta Salinas | 128 | 8 |
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