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  Royal Farm   RFV

Possible Arbovirus

No

2

Results of SALS surveys and information from the Catalogue.
Other Information
 

B
Section V - Antigenic Relationship and Lack of Relationship to Other Viruses
 
Relationship of Royal Farm virus by CF using immune serum to Royal Farm virus against antigens of 40-odd members of group B at NAMRU-3, YARU, and the Pasteur Institut, Dakar, gave titers of <16 with the exception of Langat and Powassan as shown in the table below.
 
HI and CF Tests
Royal Farm Antigen Royal Farm Immune Serum (2)
HI CF HI CF
Immune Sera Ht/Ho Ratio Ht/Ho Ratio Antigens Ht/Ho Ratio Ht/Ho Ratio


( ): Number of ip injections administered to mice
HI: serum diluted 1:5; 4 units of antigen used.
Langat (3) 40/160 1/4 16/64 1/4 Langat 20/160 1/8 16/64 1/4
Powassan (6) 20/40 1/2 64/64 1 Powassan 10/40 1/4 l6/64 1/4
 
Neutralization Tests
Royal Farm Virus a Royal Farm Serum b
Undiluted 1:10 1:16 Undiluted 1:10 1:16
Immune Serum Ht/Ho Ht/Ho Ht/Ho Virus(a) Ht/Ho Ht/Ho Ht/Ho


a Freshly prepared virus in its 10th mouse brain passage was used.
b Sera used in the neutralization tests were produced by one formalized and 2 live injections at 14-day intervals; 5 injection Langat serum was used in the 1:16 dilution neutralization test; neutralization test results as LNI in dex.
Langat (3) b 1.5/4.3 3.0/5.5 2.3/4.0 Langat 6.0/1.5 7.3/3.3 5.0/2.3
Powassan (3) 4.0/6.3 Powassan 9.3/2.3
 
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