Physicochemical
RNA, Single Strand
|
|
3 (22)
|
|
(S)
|
|
|
|
4
|
Nucleocapsid protein: 50,000 MW; glycoproteins (G1):
76,000 MW, (G2): 54,000 MW; (L) approx.200,000 MW
|
|
|
1.18-1.20 in sucrose
|
(S)
|
|
(S)
|
|
| Stability of Infectivity (effects) |
Maximum stability at pH 7.6, inactivated completely at pH 4.0
|
20%
|
<1.0 dex
|
5.8 dex
|
5%
|
<1.0 dex
|
5.8 dex
|
0.1%
|
<1.0 dex
|
5.8 dex
|
Inactivated with 0.4% formalin, 70% ethanol, 0.5% iodine and UV
irradiation
|
|
| Virion Morphology |
Spherical particles
|
100 nm
|
100 nmnm
|
90-140nm
|
Electron microscopy (4.5)
|
Surface projections and enveloped
|
|
|
| Morphogenesis |
Cytoplasm
|
|
|
|
|
|
| Hemagglutination |
Yes
|
SMB ext. by sucrose-acetone* (6); Vero E6 cell cultures
|
Goose
|
5.8-6.4
|
6.0-6.2
|
20-37dC
|
37dC
|
* HA demonstrated by this method with prototype Hantaan virus
|
PRNT, IFA, ELISA
|
* HA demonstrated by this method with prototype Hantaan virus
|
| |
