| | | Six strains were shown to be identical by cross-CF tests: AG80-226, AG80-517, AG80-785, AG80-1545, AG80-381, and AG80-504. AG80-381 (sucrose-acetone antigen) tested by CF with a battery of HIAF containing antibodies to more than 300 arboviruses and other viruses; no reactions were detected. No inhibition of hemagglutination was detected in HI tests employing HIAF for AG80-381 and antigens of viruses belonging to serogroups A, B, C, Bunyamwera, Turlock, California, and Phlebotomus fever [1] . | | NT tests were performed with five of these six isolates (AG80-785 = AG80- 1545). The results (shown below) demonstrate that, although interrelated, AG80-226, AG80-504 and AG80-381 are distinct from each other, that AG80-785 is identical with AG80-504 and that AG80-517 is a subtype of AG80-504 [1] . | | | | PRNT titer of antibody to: |
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| Strain | AG80-226 | AG80-381 | AG80-504 | AG80-785 | AG80-517 |
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| | * Reciprocal of highest dilution producing >90 plaque reduction; | | - = <10 | | AG80-226 | 2560 * | 80 | - | - | - | | AG80-381 | 320 | 320 | - | 10 | 10 | | AG80-504 | 40 | - | 640 | 640 | 160 | | AG80-785 | - | - | 640 | 1280 | 640 | | AG80-517 | 20 | - | 320 | 1280 | 1280 |
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