Physicochemical
RNA, Single Strand
|
|
1
|
no
|
38-45(S)
|
60-70%
|
20-25%
|
3-13% (2); RNA 0.7-5
|
5
|
M (MW: 150-200 x 10^3), G (MW: 66 x 10^3), N (MW: 54 x 10^3), M (MW: 27 x 10^3), NS (MW: 29-45 x 10^3) (2)
|
0
|
|
1.18 - 1.20 in sucrose
|
625 S (2)(S)
|
1.32 in CsCl
|
140 S(S)
|
|
| Stability of Infectivity (effects) |
unstable at pH 3; stable at pH 5-10 (3)
|
sensitve
|
|
|
sensitive
|
|
|
|
|
|
inactivated rapidly
|
|
| Virion Morphology |
bullet-shaped
|
180 x 75 nm
|
nm
|
nm
|
electron microscopy
|
surface projections (6-10 nm); bilayer lipid membrane (2)
|
extended 3.5 nm, helical 30-35 turns, 49 nm outer, 29 nm diameter (2)
|
|
| Morphogenesis |
cytoplasm
|
buds from plasma membrane (3)
|
extracellular and in cytoplasmic vesicles
|
not usually
|
|
|
| Hemagglutination |
Yes
|
infected BHK-21 cell cultures (4, 5)
|
goose
|
|
5.8
|
0 - 37dC
|
ice water
|
see reference 5 for optimal conditions for preparing hemagglutinin
|
CF, NT
|
see reference 5 for optimal conditions for preparing hemagglutinin
|
| |
