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Panel:   ARBank custom (n=29) (Custom)
AR Bank # 0061 Escherichia coli
Study ID: CarbaNP-30

Biosample Accession #: SAMN04014902

mCIM Result: Positive    
MLST: ST520(Pasteur), ST1408(Achtman)

Panel:  Gram Negative Carbapenemase Detection (CarbaNP)  |  Piperacillin-tazobactam +aminoglycosides for Enterobacterales (PTA) (Custom)  |  PTA - CarbaNP set only (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Amikacin 16R
Ampicillin >32R
Ampicillin/sulbactam 1>32R
Aztreonam >64R
Aztreonam/avibactam 1 20.12S
Cefazolin >8R
Cefepime >32R
Cefotaxime >64R
Cefotaxime/clavulanic acid 14---
Cefoxitin 16I
Ceftazidime 128R
Ceftazidime/avibactam 1<=0.5S
Ceftazidime/clavulanic acid 132---
Ceftriaxone >32R
Ciprofloxacin <=0.25S
Colistin 40.5I
Doripenem 4R
Ertapenem 8R
Gentamicin >16R
Imipenem 4R
Imipenem+chelators 34---
Levofloxacin <=0.12S
Meropenem 4R
Minocycline <=4S
Nitrofurantoin <=16S
Piperacillin/tazobactam 1>128R
Tetracycline >32R
Tigecycline 2<=0.5S
Tobramycin >16R
Trimethoprim/sulfamethoxazole 1>8R
S – I –R Interpretation (INT) derived from CLSI 2025 M100 S35
1 Reflects MIC of first component
2 Based on FDA break points
3 Screen for metallo-beta-lactamase production [Rasheed et al. Emerging Infectious Diseases. 2013. 19(6):870-878]
4 Clinical and PK/PD data demonstrate colistin has limited clinical efficacy, even if an intermediate result is obtained. Alternative agents are strongly preferred. Colistin should be used in combination with one or more active antimicrobial agents. Consultation with an infectious disease specialist is recommended.
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aac(6')-Ib, aadA1, aadA2, aph(3")-Ib, aph(6)-Id
Beta-lactam KPC-3, OXA-9, TEM-1A
Efflux pumps/Other EMRD, qacEdelta1
Sulfonamides sul1, sul2, sul3
Tetracyclines tet(A), tet(R)
Trimethoprim dfrA12, dfrA14
 
Disclaimer:
No disclaimer.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.



Disclaimer:

Isolate History
Date Action Performed
03/07/25INT was updated with a new value for Aztreonam/avibactam: from '---' to 'S'
03/07/25MIC was updated with a new value for Aztreonam/avibactam: from '---' to '0.12'
09/11/23INT was updated with a new value for Amikacin: from 'S' to 'R'
03/23/23Gene was added to 'qacEdelta1'
03/23/23Gene was added to 'EMRD'
03/23/23Gene was added to 'tet(R)'
03/23/23Gene was added to 'aph(6)-Id'
03/23/23Gene was added to 'aph(3")-Ib'
03/23/23Category was added: 'Efflux pumps/Other'
03/23/23Gene was removed from 'from '---' to ' strB''
03/23/23Gene was removed from 'from '---' to ' strA''
12/19/22MLST was updated: from '---' to 'ST1408(Achtman)'
12/19/22MLST was updated: from '---' to 'ST520(Pasteur)'
10/12/22INT was updated with a new value for Minocycline: from '---' to 'S'
10/12/22INT was updated with a new value for Amikacin: from 'I' to 'S'
10/12/22MIC was updated with a new value for Minocycline: from '---' to '4'
10/12/22MIC was updated with a new value for Amikacin: from '32' to '16'
10/12/22Drug was added: Minocycline
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