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Panel:   Neisseria gonorrhoeae Ciprofloxacin (NCIP)
AR Bank # 1302 Neisseria gonorrhoeae
Study ID: NCIP-18

Biosample Accession #: SRR30344928

   

Collection Year: 2022
Location: United States
Source: Urethra

MIC results provided were determined by agar dilution.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Characterization of a Neisseria gonorrhoeae Ciprofloxacin panel for an antimicrobial resistant Isolate Bank (plos.org)

Panel:  Neisseria gonorrhoeae Ciprofloxacin (NCIP)  |  Additional NCIP (Custom)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Azithromycin 2---
Cefixime 0.015S
Ceftriaxone 0.008S
Ciprofloxacin 0.5I
Gentamicin 8---
Penicillin 0.5I
Tetracycline 1I
S – I –R Interpretation (INT) derived from CLSI 2024 M100 S34
MIC results provided were determined by agar dilution
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Quinolone gyrA D95A, gyrA S91F, WT parC
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome by Illumina MiSeq. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: GC agar base and 1% defined growth supplement and/or 1% hemoglobin

GROWTH CONDITIONS
Temperature: 36°C
Atmosphere: 5% CO₂

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to GC agar

Use streak plate method to isolate single colonies

Incubate inverted plate at 36°C ± 1°C in 5% CO₂ for 20-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
11/21/24INT was updated with a new value for Azithromycin: from 'NS' to '---'
09/06/24Gene was added to Quinolone: 'WT parC'
09/06/24Gene was added to Quinolone: 'gyrA D95A'
09/06/24Gene was added to Quinolone: 'gyrA S91F'
09/06/24Gene was removed from Quinolone: 'gyrA'
09/06/24Sequence Accession # changed: from '---' to 'SRR30344928'
09/06/24INT was updated with a new value for Penicillin: from 'R' to 'I'
07/29/24New Isolate added to panel
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