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Virus Name/Prototype
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Virus Name: Puumala

Abbreviation: PUUV

Status
Probably not Arbovirus

Select Agent
No

SALS Level

SALS Basis

Other Information
USDA Permit Required, DOC Permit Required

Antigenic Group
Hantaan

Section V - Antigenic Relationship and Lack of Relationship to Other Viruses

Antigenic relations of HFRS-related viruses: PRNT were performed on Vero E6 cell monolayers with rat antisera and cell culture-adapted viruses. Approximately 100 PFU of virus were incubated with twofold dilutions of sera for 1 hour at room temperature before inoculation of 25-cm2 flasks. Inoculated cells were further incubated for 1 hour at 37/C before addition of overlay containing 0.6% agarose (Seakem ME) and 10% heated fetal bovine serum (FBS) in the nutrient mixture. Following incubation for 7 to 14 days after infection, a second overlay identical to the first except for a reduced FBS concentration (2%) and inclusion of neutral red (final concentration 0.167 mg/ml) was applied. Plaques were counted as they became visible 1 to 5 days after addition of the second overlay. Titers were expressed as the reciprocal of the highest dilution of antibody, resulting in greater than 80% reduction of approximately 100 plaques.

	Virus
Serum pools	HTN	LEE	UR	TCH	GP	SR-11	PH	PUU


Tchoupitoulas, Girard Point, and Sapporo rat viruses are unregistered.
HTN = Hantaan virus (76-118)
LEE = Human isolate from Korea
UR = Seoul virus
TCH = Tchoupitoulas
GP = Girard Point
SR-11 = Sapporo rat
PH = Prospect Hill
PUU = Puumala
HTN	4000	4000	0	160	80	40	0	0
LEE	4000	2000	20	160	160	200	0	0
UR	2000	80	4000	16000	16000	8000	160	0
TCH	2000	200	4000	32000	8000	8000	200	0
GP	200	80	2000	8000	8000	8000	200	0
SR-11	2000	20	2000	2000	2000	2000	200	0
PH	0	0	0	0	20	20	4000	0
PUU	0	0	0	0	0	0	0	1280

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