| | | The virus antigen did not react in CF testing with the following ascitic fluids: Icoaraci, Itaporanga, Karimabad, Punta Toro, Sicilian SF, Gabek Forest, but reacted with the ascitic fluid Naples SF. Antigenic relationships between Toscana and Naples SF by CF, HI, and NT (plaque method) tests were noted as follows: | | | | | Antigen or Virus |
|---|
| Ascitic fluid | Toscana | SFN |
|---|
| | CF | HI | NT | CF | HI | NT |
|---|
|
| | * Antibody titer/antigen titer | | Toscana | 256/128 * | 160 | 640 | 128/128 | 80 | 160 | | SFN | 64/64 | 40 | 0 | 256/128 | 640 | 640 |
| | At YARU [2] , it was shown by CF testing that immune serum to Toscana virus did not react with the antigens of Anhanga, Arumowot, Bujaru, Candiru, Chagres, Icoaraci, Itaporanga, Karimabad, Punta Toro, Sicilian SF, Gabek Forest. It was related to Naples SF by CF. | | Cross NT (plaque method) were performed at Pacific Research Unit Hawaii [3] using Toscana virus and hyperimmune ascitic fluids (homologous titer = >5120) against each of the following viruses and specific antisera with negative results: Aguacate, Alenquer, Anhanga, Arumowot, Buenaventura, Bujaru, Cacao, Caimito, Candiru, Chagres, Charleville, Chilibre, Gordil, Tehran, Icoaraci, Itaporanga, Karimabad, Nique, Pacui, Punta Toro, Rift Valley fever (antiserum), Rio Grande, Salehabad, Sicilian SF, Gabek Forest, Urucuri. Antigenic relationships between Toscana, Naples SF, Frijoles, and Saint Floris were noted as follows: | | | | | Virus |
|---|
| Antiserum or Ascitic Fluid | Toscana | SFN | Frijoles | Saint Floris |
|---|
|
| | Toscana | > 5120 | 10 | 40 | 80 | | SFN | 20 | 320 | 40 | 40 | | Frijoles | 0 | 0 | 10240 | 0 | | Saint Floris | 0 | 0 | 0 | 5120 |
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