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  Toscana   TOSV

Probable Arbovirus

No

2

Results of SALS surveys and information from the Catalogue.
Other Information
 

Phlebotomus Fever
Section V - Antigenic Relationship and Lack of Relationship to Other Viruses
 
The virus antigen did not react in CF testing with the following ascitic fluids: Icoaraci, Itaporanga, Karimabad, Punta Toro, Sicilian SF, Gabek Forest, but reacted with the ascitic fluid Naples SF. Antigenic relationships between Toscana and Naples SF by CF, HI, and NT (plaque method) tests were noted as follows:
 
Antigen or Virus
Ascitic fluid Toscana SFN
CF HI NT CF HI NT


* Antibody titer/antigen titer
Toscana 256/128 * 160 640 128/128 80 160
SFN 64/64 40 0 256/128 640 640
At YARU [2] , it was shown by CF testing that immune serum to Toscana virus did not react with the antigens of Anhanga, Arumowot, Bujaru, Candiru, Chagres, Icoaraci, Itaporanga, Karimabad, Punta Toro, Sicilian SF, Gabek Forest. It was related to Naples SF by CF.
Cross NT (plaque method) were performed at Pacific Research Unit Hawaii [3] using Toscana virus and hyperimmune ascitic fluids (homologous titer = >5120) against each of the following viruses and specific antisera with negative results: Aguacate, Alenquer, Anhanga, Arumowot, Buenaventura, Bujaru, Cacao, Caimito, Candiru, Chagres, Charleville, Chilibre, Gordil, Tehran, Icoaraci, Itaporanga, Karimabad, Nique, Pacui, Punta Toro, Rift Valley fever (antiserum), Rio Grande, Salehabad, Sicilian SF, Gabek Forest, Urucuri. Antigenic relationships between Toscana, Naples SF, Frijoles, and Saint Floris were noted as follows:
 
Virus
Antiserum or Ascitic Fluid Toscana SFN Frijoles Saint Floris


Toscana > 5120 10 40 80
SFN 20 320 40 40
Frijoles 0 0 10240 0
Saint Floris 0 0 0 5120
 
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