Physicochemical
RNA, Single Strand
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3
|
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29; 23; 17 s (7)(S)
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|
3
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Nucleocapsid (N): 25,000 MW;
glycoproteins (G1, G2): 75,000, 65,000 MW (4, 33, 34).
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|
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1.20 gm/cc in CsCl
|
450 s(S)
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1.31 gm/cc in CsCl (4)
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(S)
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| Stability of Infectivity (effects) |
Rel. stable at pH 4.6-11.4 (mouse brain suspension)(8)
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1:5
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1.7 dex
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5.6 dex
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|
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1:1000
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1.0 dex
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5.5 dex
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Not stabilized by MgCl2; sens. to chloroform, nonionic and ionic
detergents (4,8,9)
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| Virion Morphology |
|
95 nm
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nm
|
nm
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Electron microscopy (2-5)
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10 nm spikes, hexagonal array (2); lipid envelope (4,6)
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| Morphogenesis |
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Buds through smooth surface cytoplasmic membranes (3)
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Cisternae and vesicles of Golgi complex and endoplasmic reticulum
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| Hemagglutination |
Yes
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SMB ext. by sucrose-acetone; BHK-21 culture fluid-serum free
|
Goose
|
5.6-6.6
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5.6-5.8
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4dC, RT, 3dC
|
|
HA not readily produced from all strains
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CF, HI, NT
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HA not readily produced from all strains
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| |
