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Panel:   Shigella sonnei and Shigella flexneri (SHG)
AR Bank # 0872 Shigella flexneri
Study ID: SHG-02

Biosample Accession #: SAMN13352749

   

Collection Year: 2016
Location: USA
Source: Stool

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Shigella sonnei and Shigella flexneri (SHG)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ampicillin >32R
Azithromycin >128R
Ceftriaxone <=1S
Ciprofloxacin <=0.03S
Levofloxacin 0.06S
Nalidixic acid 2S
Tetracycline >32R
Trimethoprim/sulfamethoxazole 1<=0.5S
S – I –R Interpretation (INT) derived from CLSI 2021 M100 S31
MICs obtained by broth microdilution. Modal MIC is reported
1 Reflects MIC of first component
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
Aminoglycoside aadA1
Beta-lactam blaOXA-1, blaTEM-1B
Macrolide-Lincosamide-Streptogramin erm(B), mph(A)
Phenicols/Bicyclomycins catA1
Tetracyclines tet(B)
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using ResFinder 3.0 database. For resistance determinant detection, 90% sequence identity and 60% sequence coverage was used. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
01/03/19New Isolate added to panel
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