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Panel:   Isolates with New or Novel Antibiotic Resistance (NEW)
AR Bank # 1280 Neisseria gonorrhoeae
Study ID: NEW-37

Biosample Accession #: SAMN35332250

   

Collection Year: 2019
Location: USA
Source: Urethral swab
MLST: 1901

Isolate harbors penA allele 60.001 and has elevated MICs to ceftriaxone and cefixime

MICs obtained by broth microdilution. Modal MIC is reported.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Note: AR- 0349 Escherichia coli  has been removed from the collection and is now available at ATCC under catalog # BAA-3170

Panel:  Isolates with New or Novel Antibiotic Resistance (NEW)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Azithromycin 0.5S
Cefixime 1R
Ceftriaxone 1R
Ciprofloxacin 16R
Gentamicin 8---
Penicillin 4R
Tetracycline 2R
S – I –R Interpretation (INT) derived from CLSI 2023 M100 S33
MIC results provided were determined by agar dilution
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
 
Disclaimer:
Biosample accession numbers have been provided so that users can analyze the data on their own, if so desired.
Propagation
MEDIUM
Medium: Trypticase Soy Agar with 5% Sheep Blood (BAP)

GROWTH CONDITIONS
Temperature: 35°C
Atmosphere: Aerobic

PROPAGATION PROCEDURE

Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)

Open vial aseptically to avoid contamination

Using a sterile loop, remove a small amount of frozen isolate from the top of the vial

Aseptically transfer the loop to BAP

Use streak plate method to isolate single colonies

Incubate inverted plate at 35°C ± 2°C for 18-24 hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health. Please Note: This AR Bank panel includes isolates that are pan-resistant, meaning they are susceptible to none of the antimicrobial agents tested which may include those commonly used to treat infections with these organisms. This represents an additional risk to staff working with these organisms and should be considered as part of the receiving facility risk assessment required by the Biosafety Compliance Agreement. The increased difficulty in treatment of any infection with those isolates should also be a consideration in deciding which protocols to use and precautions to take for working with these isolates.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
03/13/24MMR was changed: from 'Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.' to 'Biosample accession numbers have been provided so that users can analyze the data on their own, if so desired.'
02/15/24Source was changed: from 'Urethra' to 'Urethral swab'
06/04/23Isolate Status changed: from 'Pending' to 'Active'
06/04/23Source was changed: from '---' to 'Urethra'
06/04/23Isolate was removed from panel
06/04/23Isolate Status changed: from 'Active' to 'Pending'
06/04/23Isolate Status changed: from 'Pending' to 'Active'
06/04/23Isolate was removed from panel
06/04/23Isolate Status changed: from 'Active' to 'Pending'
06/04/23Drug was removed: 'Aztreonam'
06/04/23INT was updated with a new value for Ceftriaxone: from 'S-R' to 'R'
06/04/23INT was updated with a new value for Cefixime: from 'S-R' to 'R'
06/04/23INT was updated with a new value for Azithromycin: from '---' to 'S'
06/04/23MIC was updated with a new value for Azithromycin: from '---' to '0.5'
06/04/23Drug was added: Azithromycin
06/04/23MMR was changed: from 'AR gene prediction was performed using ARG-ANNOT, ResFinder and NCBI (ResGANNCBI) databases accessed on 2021-05-07. AR drug classes are assigned according'' to ''these databases. This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions'
06/04/23Isolate Status changed: from 'Pending' to 'Active'
05/31/23Storage Temperature changed
05/31/23Propagation Atmosphere changed
05/31/23Growth Temperature changed
05/31/23Propagation Medium changed
05/24/23Sequence Accession # changed: from 'SAMN13975348' to 'SAMN35332250'
03/30/23INT was updated with a new value for Tetracycline: from '---' to 'R'
03/30/23MIC was updated with a new value for Tetracycline: from '---' to '2'
03/30/23INT was updated with a new value for Penicillin: from '---' to 'R'
03/30/23MIC was updated with a new value for Penicillin: from '---' to '4'
03/30/23INT was updated with a new value for Gentamicin: from '---' to '---'
03/30/23MIC was updated with a new value for Gentamicin: from '---' to '8'
03/30/23INT was updated with a new value for Ciprofloxacin: from '---' to 'R'
03/30/23MIC was updated with a new value for Ciprofloxacin: from '---' to '16'
03/30/23INT was updated with a new value for Ceftriaxone: from '---' to 'S-R'
03/30/23MIC was updated with a new value for Ceftriaxone: from '---' to '1'
03/30/23INT was updated with a new value for Cefixime: from '---' to 'S-R'
03/30/23MIC was updated with a new value for Cefixime: from '---' to '1'
03/30/23INT was updated with a new value for Aztreonam: from '---' to 'S'
03/30/23MIC was updated with a new value for Aztreonam: from '---' to '0.25'
03/30/23Drug was added: Tetracycline
03/30/23Drug was added: Penicillin
03/30/23Drug was added: Gentamicin
03/30/23Drug was added: Ciprofloxacin
03/30/23Drug was added: Ceftriaxone
03/30/23Drug was added: Cefixime
03/30/23Drug was added: Aztreonam
03/30/23Isolate Created
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