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Panel:   Clostridioides difficile EIP 2016 (CDI)
AR Bank # 1085 Clostridioides difficile
Study ID: CDI-19

Biosample Accession #: SAMN13029166

   

Collection Year: 2016
Location: USA

Ribotype: 106
MLST: ST42
TCDA: Positive
TCDB: Positive
CDTA: Negative
CDTB: Negative

A Biological Safety Cabinet is recommended for specimen handling to minimize the risk of aerosolization of spores.

MIC results provided were determined by agar dilution.

MIC results for each antimicrobial agent for an isolate may commonly be ± 1 log2 (doubling dilution) different than what is posted on the FDA & CDC AR Bank website because this is the normal technical variability of antimicrobial susceptibility testing (see J. H. Jorgensen. 1993. J Clin Microbiol. Vol 31[11]: 2841-2844).

Panel:  Clostridioides difficile EIP 2016 (CDI)


MIC (μg/ml) Results and Interpretation
Drug MIC (μg/ml) INT
Ceftriaxone 64---
Clindamycin 8---
Meropenem 2---
Metronidazole 0.25S
Moxifloxacin 2---
Vancomycin 0.5WT
S – I –R Interpretation (INT) derived from CLSI 2021 M100 S31
WT/NWT interpretations are based on a CLSI established epidemiological cut-off value
MIC results provided were determined by agar dilution
Device manufacturers and users of FDA cleared devices shall consult the FDA’s Antibacterial Susceptibility Test Interpretive Criteria       website for breakpoints recognized or recommended by FDA, and for information regarding FDA exceptions or additions to the applicable, recognized consensus standard.
Molecular Mechanisms of Resistance
CategoryGene
 
Disclaimer:
The resistance mechanisms listed were identified by analysis of whole genome sequence using the ResFinder database (last updated June 2, 2016 and accessed on October 25, 2016). This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of the ResFinder database or other antimicrobial resistance gene databases. Biosample accession numbers have been provided so that users can analyze the data on their own if so desired.
Propagation
MEDIUM
Medium: CDC Anaerobic Blood Agar (ANA BAP) or Brucella Blood Agar (BBA)

GROWTH CONDITIONS
Temperature: 37°C
Atmosphere: Anaerobic

PROPAGATION PROCEDURE
Remove the sample vial to a container with dry ice or a freezer block. Keep vial on ice or block. (Do not let vial content thaw)
Open vial aseptically to avoid contamination
Using a sterile loop, remove a small amount of frozen isolate from the top of the vial
Aseptically transfer the loop to ANA BAP or BBA
Use streak plate method to isolate single colonies
Incubate inverted plate at 35⁰C ± 2°C for 24hrs.
Storage Temperature & Biosafety
STORAGE TEMPERATURE: -70°C

BIOSAFETY LEVEL: 2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the current publication of 'BioSafety in Microbiological and Biomedical Laboratories' from the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, and National Institutes of Health.

Disclaimer:
This product is sent with the condition that you are responsible for its safe storage, handling, and use. All materials are the property of the Centers for Disease Control and Prevention (CDC) and have been made available on behalf of the Food and Drug Administration (FDA). This material is not for use in human subjects and may not be redistributed. While CDC uses reasonable efforts to include accurate and up-to-date information on this product sheet, CDC makes no warranties or representations as to its accuracy. CDC is not liable for damages arising from the misidentification or misrepresentation of cultures. Please refer to the Standard Letter Agreement (SLA) for further details regarding the use of this product.
Isolate History
Date Action Performed
10/04/19New Isolate added to panel
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