ID#: 2189
Caption:
Under a magnification of 1000X, this photomicrograph of a sputum smear, processed using a fluorescence acid-fast staining method that implements acridine orange fluorescent dye as a counterstain, revealed the presence of a Mycobacterium tuberculosis bacterium. M. tuberculosis is an acid-fast bacterium (AFB), and is therefore, undetectable when stained using a Gram stain technique. However, using the Smithwick method, which employs acridine orange as the specific dye, the M. tuberculosis bacteria glow yellow under ultraviolet light microscopy.
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Content Provider(s): CDC/ Ronald W. Smithwick
Creation Date: 1971
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