PPE-Info - Standard Details

ASTM D5712 - 15(2020)

ASTM International (ASTM)

Title

"Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method "

Description

This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

Standard Type(s)

Test Method

A definitive procedure that produces a test result: Examples of test methods include, but are not limited to: identification, measurement, and evaluation of one or more qualities, characteristics, or properties. A precision and bias statement shall be reported at the end of a test method.

Personal Protective Equipment

Hand and Arm Protection

All equipment and/or accessories (whether or not detachable) designed and manufactured specifically to protect the arm and/or the hand this includes all garments protecting the hand or part of the hand, including gloves, fingerless gloves, mittens, garments protecting the fingers only or the palm only, etc. Potential hazards include skin absorption of harmful substances, chemical or thermal burns, electrical dangers, bruises, abrasions, cuts, punctures, fractures and amputations.

Medical gloves

Hazards

Biological

Biological

Standard References

Referenced Standard

ASTM D3577 - 19(2023) - Standard Specification for Rubber Surgical Gloves

ASTM D3578 - 19(2023) - Standard Specification for Rubber Examination Gloves

Conformity Assessment Notes

Testing procedures required, please see the full standard for complete details.

Comments

Additional References:

ASTM Standards

D3577 Specification for Rubber Surgical Gloves
D3578 Specification for Rubber Examination Gloves
D4483 Practice for Evaluating Precision for Test Method Standards in the Rubber and Carbon Black Manufacturing Industries

Testing Laboratories

Intertek - Cortland, NY, 13045

Contact

Phone Number: (607) 753-6711
Email: jason.allen@intertek.com

Accreditations

ISO 17025

Scope of Accreditation

ANSI/ISEA Z87.1-2020 , ASTM F2412 - 18a , ASTM D120 - 22 , ASTM D3577 - 19(2023) , ASTM D3578 - 19(2023) , ASTM D5250 - 19(2023) , ASTM D5712 - 15(2020) , ASTM D6319 - 19(2023) , ASTM D6978 - 05(2023) , ASTM D7246 - 06(2021) , ASTM F1446 - 20 , ANSI/AAMI PB70:2022 , ASTM F1342 / F1342M - 05(2022) , ASTM F1359 / F1359M - 22 , ASTM F1670 / F1670M - 17a , ASTM F1671 / F1671M - 22 , ASTM F1790 / F1790M - 15 (Reapproved 2021) , ASTM F2588 - 12(2020) , ASTM F739 - 20 , ASTM F903 - 18 , ASTM F1862 / F1862M - 17 , ASTM F2100 - 23 , ASTM F2101 - 23 , ASTM F2299 / F2299M - 24 , ANSI/AAMI PB70 - Class 4 , ANSI/AAMI PB70 - Class 3

Akron Rubber Development Laboratory, Inc. - Akron, OH, 44322-1016

Notes

Accredited Gloves Testing Methods ASTM Testing Methods

ASTM D6319 - Standard Specification for Nitrile Examination Gloves for Medical Application
ASTM D6978 - Standard Practice for Assessment of Resistance of Medical Gloves to Permeation by Chemotherapy Drugs
ASTM D7558 - Colorimetric/Spectrophotometric Procedure to Quantify Extractable Chemical Dialkyldithiocarbamate, Thiuram and Mercaptobenzothiazole Accelerators in Natural Rubber Latex and Nitrile Gloves
ASTM D120 — Standard Specification for Rubber Insulating Gloves
ASTM D3577 — Standard Specification for Rubber Surgical Gloves
ASTM D3578 — Standard Specification for Rubber Examination Gloves
ASTM D5151 — Standard Test Method for Detection of Holes in Medical Gloves
ASTM D6124 — Standard Test Method for Residual Powder on Medical Gloves
ASTM D5250 - Standard Specification for Poly (Vinyl Chloride) Gloves for Medical Application
ARDL 2140 - Lubricant Compatibility Testing on Gloves
ASTM D5712 - Analysis of Aqueous Extractable Protein in Latex, Natural Robber and Elastomeric Products Using the Modified Lowry Method
ASTM D6499 - Immunological Measurement of Antigenic Protein in Natural Rubber and Its Products
ASTM F739 — Standard Test Method for Permeation of Liquids and Gases Through Protective Clothing Materials Under Conditions of Continuous Contact
ASTM F1383 - Permeation of Liquids and Gases Through Protective Clothing Materials Under Conditions of Intermittent Contact
ASTM F1670 - Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Synthetic Blood
ASTM F1671/F1671M — Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System
ASTM F903 — Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Liquids
ASTM E96/E96M Water Vapor Transmission of Materials
ASTM D6977 — Standard Specification for Polychloroprene Examination Gloves for medical Application
ASTM D7246 — Standard Test Method for Detection of Holes in Polyethylene Food Service Gloves
ASTM D7329 — Standard Specification for Food Preparation and Food Handling Glove