National Health and Nutrition Examination Survey

NHANES 2009-2012 Oral Microbiome

Data documentation, Data files, and Variable/Annotation files

Oral microbiome testing was performed using oral rinse samples collected from NHANES participants in two consecutive NHANES cycles, 2009-2010 and 2011-2012. Results from the oral microbiome testing encompass classification of microbial sequences into amplicon sequence variants, which were used to generate alpha-diversity metrics, beta-diversity matrices, and relative abundance/read count tables for each taxonomic level from phylum to genus.

For information about oral microbiome data files, review Oral Microbiome Data Documentation [PDF - 818 KB] and the variable/annotation files below. The documentation describes eligible samples, laboratory procedures, bioinformatics procedures, and analytic notes; plus it provides a dictionary of terms and a process flow chart.

All public use data files are available below.

Questions: Email NHANESGenetics@cdc.gov.txt

File Name Data File Variable/Annotation File Date Published
Alpha Diversity
Alpha Diversity - DADA2 Remove unassigned bacteria dada2rb-alpha.txt   October 2022
Alpha Diversity variable list - DADA2 Remove unassigned bacteria   dada2rb-variablelist-alpha.txt October 2022
Alpha Diversity - DADA2 Remove SV1032 dada2rsv-alpha.txt   October 2022
Alpha Diversity variable list - DADA2 Remove SV1032   dada2rsv-variablelist-alpha.txt October 2022
Beta Diversity
Beta Diversity Bray-Curtis - DADA2 Remove unassigned bacteria dada2rb-braycurtis-beta.txt   October 2022
Beta Diversity unweighted Unifrac - DADA2 Remove unassigned bacteria dada2rb-unwunifrac-beta.txt   October 2022
Beta Diversity weighted Unifrac - DADA2 Remove unassigned bacteria dada2rb-wunifrac-beta.txt   October 2022
Beta Diversity Bray-Curtis - DADA2 Remove SV1032 dada2rsv-braycurtis-beta.txt   October 2022
Beta Diversity unweighted Unifrac - DADA2 Remove SV1032 dada2rsv-unwunifrac-beta.txt   October 2022
Beta Diversity weighted Unifrac - DADA2 Remove SV1032 dada2rsv-wunifrac-beta.txt   October 2022
Relative Abundance and Read Count
Read Count Phylum - DADA2 Remove unassigned bacteria dada2rb-phylum-count.txt   November 2024
Relative Abundance Phylum - DADA2 Remove unassigned bacteria dada2rb-phylum-relative.txt   November 2024
Read Count Class - DADA2 Remove unassigned bacteria dada2rb-class-count.txt   November 2024
Relative Abundance Class - DADA2 Remove unassigned bacteria dada2rb-class-relative.txt   November 2024
Read Count Order - DADA2 Remove unassigned bacteria dada2rb-order-count.txt   November 2024
Relative Abundance Order - DADA2 Remove unassigned bacteria dada2rb-order-relative.txt   November 2024
Read Count Family - DADA2 Remove unassigned bacteria dada2rb-family-count.txt   November 2024
Relative Abundance Family - DADA2 Remove unassigned bacteria dada2rb-family-relative.txt   November 2024
Read Count Genus - DADA2 Remove unassigned bacteria dada2rb-genus-count.txt   November 2024
Relative Abundance Genus - DADA2 Remove unassigned bacteria dada2rb-genus-relative.txt   November 2024
Taxonomy Annotation - DADA2 Remove unassigned bacteria   dada2rb-taxonomy-annotate.txt October 2022
Read Count Phylum - DADA2 Remove SV1032 dada2rsv-phylum-count.txt   November 2024
Relative Abundance Phylum - DADA2 Remove SV1032 dada2rsv-phylum-relative.txt   November 2024
Read Count Class - DADA2 Remove SV1032 dada2rsv-class-count.txt   November 2024
Relative Abundance Class - DADA2 Remove SV1032 dada2rsv-class-relative.txt   November 2024
Read Count Order - DADA2 Remove SV1032 dada2rsv-order-count.txt   November 2024
Relative Abundance Order - DADA2 Remove SV1032 dada2rsv-order-relative.txt   November 2024
Read Count Family - DADA2 Remove SV1032 dada2rsv-family-count.txt   November 2024
Relative Abundance Family - DADA2 Remove SV1032 dada2rsv-family-relative.txt   November 2024
Read Count Genus - DADA2 Remove SV1032 dada2rsv-genus-count.txt   November 2024
Relative Abundance Genus - DADA2 Remove SV1032 dada2rsv-genus-relative.txt   November 2024
Read Count Phylum - DADA2 Remove SV1032   dada2rsv-taxonomy-annotate.txt October 2022