National Health and Nutrition Examination Survey

NHANES 2009-2012 Oral Microbiome

Data documentation, Data files, and Variable/Annotation files

Oral microbiome testing was performed using oral rinse samples collected from NHANES participants in two consecutive NHANES cycles, 2009-2010 and 2011-2012. Results from the oral microbiome testing encompass classification of microbial sequences into amplicon sequence variants, which were used to generate alpha-diversity metrics, beta-diversity matrices, and relative abundance/read count tables for each taxonomic level from phylum to genus.

For information about oral microbiome data files, review Oral Microbiome Data Documentation [PDF - 270 KB] and the variable/annotation files below. The documentation describes eligible samples, laboratory procedures, bioinformatics procedures, and analytic notes; plus it provides a dictionary of terms and a process flow chart.

All public use data files are available. Restricted data files are indicated as such in the table below. Proposals to analyze restricted oral microbiome data files may be submitted to the NCHS Research Data Center (RDC). For additional information on restricted-use data proposal submission guidelines, please go to the RDC website.

Questions: Email NHANESGenetics@cdc.gov

Data File Name Data File Variable/Annotation File Date Published
Alpha Diversity - DADA2 Remove unassigned bacteria dada2rb-alpha [TXT - 14.4 MB] dada2rb-variablelist-alpha [TXT - 20.6 KB] October 2022
Alpha Diversity - DADA2 Remove SV1032 dada2rsv-alpha [TXT - 14.4 MB] dada2rsv-variablelist-alpha [TXT - 20.8 KB] October 2022
Beta Diversity Bray-Curtis - DADA2 Remove unassigned bacteria dada2rb-braycurtis-beta [TXT - 574 MB]   October 2022
Beta Diversity unweighted Unifrac - DADA2 Remove unassigned bacteria dada2rb-unwunifrac-beta [TXT - 990 MB]   October 2022
Beta Diversity weighted Unifrac - DADA2 Remove unassigned bacteria dada2rb-wunifrac-beta [TXT - 990 MB]   October 2022
Beta Diversity Bray-Curtis - DADA2 Remove SV1032 dada2rsv-braycurtis-beta [TXT - 574 MB]   October 2022
Beta Diversity unweighted Unifrac - DADA2 Remove SV1032 dada2rsv-unwunifrac-beta [TXT - 991 MB]   October 2022
Beta Diversity weighted Unifrac - DADA2 Remove SV1032 dada2rsv-wunifrac-beta [TXT - 991 MB]   October 2022
Read Count Phylum - DADA2 Remove unassigned bacteriadada2rb-phylum-count.txt [Restricted] dada2rb-taxonomy-annotate [TXT - 176 KB] October 2022
Relative Abundance Phylum - DADA2 Remove unassigned bacteria dada2rb-phylum-relative.txt [Restricted] October 2022
Read Count Class - DADA2 Remove unassigned bacteria dada2rb-class-count.txt [Restricted] October 2022
Relative Abundance Class - DADA2 Remove unassigned bacteria dada2rb-class-relative.txt [Restricted] October 2022
Read Count Order - DADA2 Remove unassigned bacteria dada2rb-class-count.txt [Restricted] October 2022
Relative Abundance Order - DADA2 Remove unassigned bacteria dada2rb-class-relative.txt [Restricted] October 2022
Read Count Family - DADA2 Remove unassigned bacteria dada2rb-family-count.txt [Restricted] October 2022
Relative Abundance Family - DADA2 Remove unassigned bacteria dada2rb-family-relative.txt [Restricted] October 2022
Read Count Genus - DADA2 Remove unassigned bacteria dada2rb-genus-count.txt [Restricted] October 2022
Relative Abundance Genus - DADA2 Remove unassigned bacteria dada2rb-genus-relative.txt [Restricted] October 2022
Read Count Phylum - DADA2 Remove SV1032 dada2rsv-genus-phylum.txt [Restricted] dada2rsv-taxonomy-annotate [TXT - 179 KB] October 2022
Relative Abundance Phylum - DADA2 Remove SV1032 dada2rsv-phylum-relative.txt [Restricted] October 2022
Read Count Class - DADA2 Remove SV1032 dada2rsv-class-count.txt [Restricted] October 2022
Relative Abundance Class - DADA2 Remove SV1032 dada2rsv-class-relative.txt [Restricted] October 2022
Read Count Order - DADA2 Remove SV1032 dada2rsv-order-count.txt [Restricted] October 2022
Relative Abundance Order - DADA2 Remove SV1032 dada2rsv-order-relative.txt [Restricted] October 2022
Read Count Family - DADA2 Remove SV1032 dada2rsv-family-count.txt [Restricted] October 2022
Relative Abundance Family - DADA2 Remove SV1032 dada2rsv-family-relative.txt [Restricted] October 2022
Read Count Genus - DADA2 Remove SV1032 dada2rsv-genus-count.txt [Restricted] October 2022
Relative Abundance Genus - DADA2 Remove SV1032 dada2rsv-genus-relative.txt [Restricted] October 2022