Lab Method Name |
Documentation |
Release Date |
Alanine Amino Transferase (ALT) |
[PDF - 244 KB]
|
September 2011 |
Albumin |
[PDF - 263 KB]
|
September 2011 |
Alkaline Phosphatase (ALP) |
[PDF - 237 KB]
|
September 2011 |
Apolipoprotein B |
[PDF - 363 KB]
|
September 2011 |
Arsenobetaine, Arsenocholine, Trimethylarsine oxide, Monomethylarsonic acid, Dimethylarsinic acid, Arsenous (III) acid, Arsenic (V) acid, Total Arsenic |
[PDF - 2.37 MB]
|
September 2011 |
Aspartate Aminotransferase (AST) |
[PDF - 235 KB]
|
September 2011 |
Bicarbonate (HCO3) |
[PDF - 416 KB]
|
September 2011 |
Bilirubin |
[PDF - 238 KB]
|
September 2011 |
Blood Urea Nitrogen (BUN) |
[PDF - 238 KB]
|
September 2011 |
C-Reactive Protein |
[PDF - 324 KB]
|
September 2011 |
Caffeine and caffeine metabolites |
[PDF - 1.3 MB]
|
July 2014 |
Chlamydia trachomatis |
[PDF - 121 KB]
|
September 2011 |
Chloride |
[PDF - 278 KB]
|
September 2011 |
Cholesterol |
[PDF - 256 KB]
|
September 2011 |
Complete Blood Count |
[PDF - 5.48 MB]
|
September 2011 |
Complex Prostate-Specific Antigen (PSA) |
[PDF - 266 KB]
|
September 2011 |
Cotinine |
[PDF - 840 KB]
|
September 2011 |
Creatinine |
[PDF - 250 KB]
|
September 2011 |
Endomysial Antibody Assay |
[PDF - 42 KB]
|
September 2012 |
Environmental Pesticides |
[PDF - 603 KB]
|
August 2012 |
Environmental Phenol |
[PDF - 865 KB]
|
August 2012 |
Fasting Glucose |
[PDF - 224 KB]
|
September 2011 |
Ferritin |
[PDF - 195 KB]
|
February 2012 |
Free Prostate-Specific Antigen (PSA) |
[PDF - 277 KB]
|
September 2011 |
Free Thyroxine (Free T4) (Collaborative Laboratory Services ) |
[PDF - 305 KB]
|
September 2011 |
Free Thyroxine (Free T4) (University of Washington Medical Center) |
[PDF - 312 KB]
|
September 2011 |
Free Triiodothyronine, Free T3 (Collaborative Laboratory Services) |
[PDF - 295 KB]
|
September 2011 |
Free Triiodothyronine, Free T3 (University of Washington Medical Center) |
[PDF - 255 KB]
|
September 2011 |
Gamma-Glutamyl Transferase (GGT) |
[PDF - 270 KB]
|
September 2011 |
Glucose |
[PDF - 234 KB]
|
September 2011 |
Glycohemoglobin |
[PDF - 248 KB]
|
September 2011 |
HDL- Cholesterol |
[PDF - 314 KB]
|
September 2011 |
HIV Antibody / HIV Western Blot Confirmatory Test |
[PDF - 172 KB]
|
September 2011 |
Heavy Metals - Antimony, Barium, Beryllium, Cadmium, Cesium, Cobalt, Lead, Molybdenum, Platinum, Thallium, Tungsten, and Uranium |
[PDF - 3.02 MB]
|
September 2011 |
Hepatitis A Antibody |
[PDF - 666 KB]
|
September 2011 |
Hepatitis B Core Antibody |
[PDF - 828 KB]
|
September 2011 |
Hepatitis B Surface Antibody (anti-HBs) |
[PDF - 706 KB]
|
September 2011 |
Hepatitis B Surface Antigen (HBsAg) |
[PDF - 870 KB]
|
September 2011 |
Hepatitis C Antibody / Hepatitis C Confirmatory Test (Anti-HCV) |
[PDF - 805 KB]
|
September 2011 |
Hepatitis C Genotype |
[PDF - 308 KB]
|
September 2011 |
Hepatitis C RNA Qualitative Assay for Hepatitis C Virus |
[PDF - 218 KB]
|
September 2011 |
Hepatitis D Antibody |
[PDF - 106 KB]
|
September 2011 |
Hepatitis E Antibody (IgG) |
[PDF - 122 KB]
|
January 2012 |
Hepatitis E Antibody (IgM) |
[PDF - 119 KB]
|
January 2012 |
Herpes Simplex Virus Type 1 & 2 |
[PDF - 81 KB]
|
September 2011 |
Human Papillomavirus |
[PDF - 85 KB]
|
December 2013 |
Human Papillomavirus |
[PDF - 154 KB]
|
September 2011 |
Insulin - Elecsys 2010 Immunoassay |
[PDF - 409 KB]
|
August 2016 |
Insulin - Mercodia ELISA |
[PDF - 841 KB]
|
August 2016 |
Iodine & Mercury |
[PDF - 779 KB]
|
September 2011 |
Iron |
[PDF - 244 KB]
|
September 2011 |
Lactate Dehydrogenase |
[PDF - 249 KB]
|
September 2011 |
Lead Cadmium Mercury |
[PDF - 898 KB]
|
September 2011 |
Measles Antibody |
[PDF - 117 KB]
|
September 2011 |
Mercury |
[PDF - 571 KB]
|
September 2011 |
Monohydroxy-Polycyclic Aromatic Hydrocarbons |
[PDF - 1.6 MB]
|
August 2015 |
Mumps Antibody |
[PDF - 104 KB]
|
September 2011 |
N,N-diethyl-3-methylbenzamide, N,Ndiethyl-3-hydroxymethylbenzamide, and 3-diethyl-carbamoyl benzoic acid |
[PDF - 577 KB]
|
January 2016 |
Osmolality |
[PDF - 502 KB]
|
September 2011 |
Perchlorate, Nitrate, and Thiocyanate |
[PDF - 443 KB]
|
April 2014 |
Phosphorus |
[PDF - 239 KB]
|
September 2011 |
Polyfluoroalkyl chemicals |
[PDF - 3.18 MB]
|
December 2011 |
Potassium |
[PDF - 253 KB]
|
September 2011 |
Rubella Antibody |
[PDF - 126 KB]
|
September 2011 |
Sodium |
[PDF - 292 KB]
|
September 2011 |
Specific Organophosphorous Pesticides, Synthetic Pyrethroids, and Select Herbicides (Universal Pesticides) |
[PDF - 1.1 MB]
|
August 2014 |
Thyroglobulin Antibodies (Collaborative Laboratory Services) |
[PDF - 254 KB]
|
September 2011 |
Thyroglobulin Antibodies (University of Washington Medical Center) |
[PDF - 335 KB]
|
September 2011 |
Thyroglobulin, (TGN) (Collaborative Laboratory Services) |
[PDF - 254 KB]
|
September 2011 |
Thyroglobulin, (TGN) (University of Washington Medical Center) |
[PDF - 287 KB]
|
September 2011 |
Thyroid Peroxidase Antibodies (Collaborative Laboratory Services) |
[PDF - 285 KB]
|
September 2011 |
Thyroid Peroxidase Antibodies (University of Washington Medical Center) |
[PDF - 335 KB]
|
September 2011 |
Thyroid Stimulating Hormone (TSH) (Collaborative Laboratory Services ) |
[PDF - 240 KB]
|
September 2011 |
Thyroid Stimulating Hormone (TSH) (University of Washington Medical Center) |
[PDF - 320 KB]
|
September 2011 |
Tissue Transglutaminase Assay (IgA) |
[PDF - 42 KB]
|
September 2011 |
Total Calcium |
[PDF - 245 KB]
|
September 2011 |
Total Cholesterol |
[PDF - 337 KB]
|
September 2011 |
Total Folate |
[PDF - 1.42 MB]
|
October 2011 |
Total Prostate-Specific Antigen (PSA) |
[PDF - 264 KB]
|
September 2011 |
Total Protein |
[PDF - 296 KB]
|
September 2011 |
Total Thyroxine, Total T4 (Collaborative Laboratory Services) |
[PDF - 345 KB]
|
September 2011 |
Total Thyroxine, Total T4 (University of Washington Medical Center) |
[PDF - 338 KB]
|
September 2011 |
Total Triiodothyronine, Total T3 (Collaborative Laboratory Services) |
[PDF - 326 KB]
|
September 2011 |
Total Triiodothyronine, Total T3 (University of Washington Medical Center) |
[PDF - 288 KB]
|
September 2011 |
Total and Inorganic Mercury |
[PDF - 775 KB]
|
September 2011 |
Trans Fatty Acids |
[PDF - 854 KB]
|
April 2016 |
Transferrin Receptor |
[PDF - 195 KB]
|
February 2012 |
Triglycerides (Beckman UniCel® DxC800 Synchron) |
[PDF - 271 KB]
|
September 2011 |
Triglycerides (Roche/Hitachi Modular P Chemistry Analyzer) |
[PDF - 302 KB]
|
September 2011 |
Two Hour Glucose (OGTT) |
[PDF - 231 KB]
|
September 2011 |
Uric Acid |
[PDF - 249 KB]
|
September 2011 |
Urinary Albumin |
[PDF - 492 KB]
|
September 2011 |
Urinary Creatinine |
[PDF - 250 KB]
|
September 2011 |
Urinary Phthalates |
[PDF - 1,321 KB]
|
September 2012 |
Urinary Phytoestrogens |
[PDF - 760 KB]
|
January 2013 |
Varicella Antibody (gp) |
[PDF - 86 KB]
|
September 2011 |
Varicella Antibody (whole cell) |
[PDF - 88 KB]
|
September 2011 |
Vitamin B6 (pyridoxal 5'-phosphate; 4-pyridoxic acid) |
[PDF - 984 KB]
|
October 2015 |
Vitamin D (25-Hydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3) |
[PDF - 613 KB]
|
October 2015 |
Automated Data Collection
Each MEC examination had computerized data collection. The examination component data entry systems had built-in quality control checks. Unusual data entries were flagged and a message was sent to the technicians. The technicians were required to either verify the original entry or edit the response. At the end of each examination session, data were sent to a central survey database.
Spanish Translations of Protocol Instructions
All NHANES brochures, consent forms, hand cards, protocols, and correspondence were produced in English and Spanish. All materials were translated as part of the NHANES, Institutional Review Board (IRB) approved, forward translation process. Extensive training was completed with MEC staff to ensure the quality and comparability of staff interactions with Spanish-speaking respondents.
Technician Training
The qualifications for MEC laboratory, physician, dental, and health technician staff are described in the component training manuals. All MEC staff completed the requirements for safety, subject privacy and confidentiality, and cardiopulmonary resuscitation training. In addition, all staff completed component-specific training to learn the standardized NHANES protocol for each of the examinations they performed.
Quality Control Monitoring- General Procedures
Quality control measures included equipment calibration and observations of examinations in the field. Experienced trainers and observers monitored technician performance in the field. Periodic retraining sessions were conducted with the survey staff.
For a subset of examination components (e.g., body measurements, blood pressure and the dental examination), a "gold standard" examiner performed the second examinations during a site visit.
Consistency Checks
An extensive series of quality assurance and quality control analyses were completed for each examination component. The MEC examination component training manuals, provided with these data files, describe the measures taken in greater detail.
Data Preparation
Routine data preparation procedures included a review of frequency data, outliers, and technician notes. Analysts should review the data reported for each component or laboratory assay, prior to beginning data analyses. Analysts should examine the data spread and consider whether or not it is appropriate to include or exclude extreme values in a given analysis.